Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

Marie Willaing Johannsen; Jesper Wengel; Michael Chr. Wamberg; Lia Crispino; Neerja Kalra

Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

Marie Willaing Johannsen, Jesper Wengel, Michael Chr. Wamberg, Lia Crispino, Neerja Kalra

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning

Abstrakt

The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.

Originalsprog	Engelsk
Publikationsdato	2010
Antal sider	1
Status	Udgivet - 2010

Bibliografisk note

Abstracts of Papers, 239th ACS National Meeting, San Francisco, CA, United States, March 21-25, 2010
Pages CARB-45

1 Organisering af eller deltagelse i workshop, kursus, seminar eller lignende

239th American Chemical Society National meeting
Marie Willaing Johannsen (Deltager)
21. mar. 2010 → 25. mar. 2010
Aktivitet: Deltagelse i faglig begivenhed › Organisering af eller deltagelse i workshop, kursus, seminar eller lignende

Citationsformater

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title = "Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability",

abstract = "The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.",

author = "Johannsen, {Marie Willaing} and Jesper Wengel and Wamberg, {Michael Chr.} and Lia Crispino and Neerja Kalra",

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TY - ABST

T1 - Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

AU - Johannsen, Marie Willaing

AU - Wengel, Jesper

AU - Wamberg, Michael Chr.

AU - Crispino, Lia

AU - Kalra, Neerja

N1 - Abstracts of Papers, 239th ACS National Meeting, San Francisco, CA, United States, March 21-25, 2010 Pages CARB-45

PY - 2010

Y1 - 2010

N2 - The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.

AB - The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.

M3 - Conference abstract for conference

ER -

Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

Abstrakt

Bibliografisk note

239th American Chemical Society National meeting

Citationsformater