Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

Marie Willaing Johannsen, Jesper Wengel, Michael Chr. Wamberg, Lia Crispino, Neerja Kalra

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskning

Abstrakt

The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.
OriginalsprogEngelsk
Publikationsdato2010
Antal sider1
StatusUdgivet - 2010

Bibliografisk note

Abstracts of Papers, 239th ACS National Meeting, San Francisco, CA, United States, March 21-25, 2010
Pages CARB-45

Citationsformater