All-trans retinoic acid increases oxidative metabolism in mature adipocytes

Josep Mercader, Lise Madsen, Francisco Felipe, Andreu Palou, Karsten Kristiansen, M Luisa Bonet

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2007-null
OriginalsprogEngelsk
TidsskriftCellular Physiology and Biochemistry
Vol/bind20
Udgave nummer6
Sider (fra-til)1061-1072
Antal sider11
ISSN1015-8987
DOI
StatusUdgivet - 1. jan. 2007

Fingeraftryk

Adipocytes
PPAR gamma
Fatty Acids
Retinoblastoma Protein
White Adipose Tissue
Lipid Metabolism
Adipose Tissue
Rodentia
Fasting
Fats
Messenger RNA
Enzymes
Proteins

Citer dette

Mercader, J., Madsen, L., Felipe, F., Palou, A., Kristiansen, K., & Bonet, M. L. (2007). All-trans retinoic acid increases oxidative metabolism in mature adipocytes. Cellular Physiology and Biochemistry, 20(6), 1061-1072. https://doi.org/10.1159/0000110717
Mercader, Josep ; Madsen, Lise ; Felipe, Francisco ; Palou, Andreu ; Kristiansen, Karsten ; Bonet, M Luisa. / All-trans retinoic acid increases oxidative metabolism in mature adipocytes. I: Cellular Physiology and Biochemistry. 2007 ; Bind 20, Nr. 6. s. 1061-1072.
@article{612ffe80de1f11dc860c000ea68e967b,
title = "All-trans retinoic acid increases oxidative metabolism in mature adipocytes",
abstract = "BACKGROUND/AIMS: In rodents, retinoic acid (RA) treatment favors loss of body fat mass and the acquisition of brown fat features in white fat depots. In this work, we sought to examine to what extent these RA effects are cell autonomous or dependent on systemic factors. METHODS: Parameters of lipid metabolism and related gene expression were analyzed in differentiated 3T3-L1 adipocytes after exposure to RA or vehicle. RESULTS: Treatment with RA resulted in decreased cellular triacylglycerol content and increased basal lipolysis and fatty acid oxidation rate. At the mRNA level, RA treatment led to a reduced expression of adipogenic/lipogenic transcription factors (peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, rexinoid receptor alpha) and two purported suppressors of lipolysis and oxidative metabolism (CIDEA and receptor-interacting protein 140), and to an increased expression of proteins favoring fat oxidation (peroxisome proliferator-activated receptor gamma coactivator-1alpha, uncoupling protein 2, fasting-induced adipose factor, enzymes of mitochondrial fatty acid oxidation). These changes paralleled inactivation of the retinoblastoma protein and were preceded by an early RA-induced phosphorylation of p38 mitogen-activated protein kinase. UCP1 expression was not induced. CONCLUSION: The results indicate that RA directly favors remodeling of mature 3T3-L1 adipocytes in culture toward increased oxidative metabolism.",
keywords = "3T3-L1 Cells, Adipocytes, Adipogenesis, Animals, Fatty Acids, Gene Expression Regulation, Ion Channels, Lipid Metabolism, Lipogenesis, Mice, Mitochondrial Proteins, Oxidation-Reduction, Phosphorylation, Retinoblastoma Protein, Transcription Factors, Tretinoin, Triglycerides, p38 Mitogen-Activated Protein Kinases",
author = "Josep Mercader and Lise Madsen and Francisco Felipe and Andreu Palou and Karsten Kristiansen and Bonet, {M Luisa}",
year = "2007",
month = "1",
day = "1",
doi = "10.1159/0000110717",
language = "English",
volume = "20",
pages = "1061--1072",
journal = "Cellular Physiology and Biochemistry",
issn = "1015-8987",
publisher = "S. Karger AG",
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Mercader, J, Madsen, L, Felipe, F, Palou, A, Kristiansen, K & Bonet, ML 2007, 'All-trans retinoic acid increases oxidative metabolism in mature adipocytes', Cellular Physiology and Biochemistry, bind 20, nr. 6, s. 1061-1072. https://doi.org/10.1159/0000110717

All-trans retinoic acid increases oxidative metabolism in mature adipocytes. / Mercader, Josep; Madsen, Lise; Felipe, Francisco; Palou, Andreu; Kristiansen, Karsten; Bonet, M Luisa.

I: Cellular Physiology and Biochemistry, Bind 20, Nr. 6, 01.01.2007, s. 1061-1072.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - All-trans retinoic acid increases oxidative metabolism in mature adipocytes

AU - Mercader, Josep

AU - Madsen, Lise

AU - Felipe, Francisco

AU - Palou, Andreu

AU - Kristiansen, Karsten

AU - Bonet, M Luisa

PY - 2007/1/1

Y1 - 2007/1/1

N2 - BACKGROUND/AIMS: In rodents, retinoic acid (RA) treatment favors loss of body fat mass and the acquisition of brown fat features in white fat depots. In this work, we sought to examine to what extent these RA effects are cell autonomous or dependent on systemic factors. METHODS: Parameters of lipid metabolism and related gene expression were analyzed in differentiated 3T3-L1 adipocytes after exposure to RA or vehicle. RESULTS: Treatment with RA resulted in decreased cellular triacylglycerol content and increased basal lipolysis and fatty acid oxidation rate. At the mRNA level, RA treatment led to a reduced expression of adipogenic/lipogenic transcription factors (peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, rexinoid receptor alpha) and two purported suppressors of lipolysis and oxidative metabolism (CIDEA and receptor-interacting protein 140), and to an increased expression of proteins favoring fat oxidation (peroxisome proliferator-activated receptor gamma coactivator-1alpha, uncoupling protein 2, fasting-induced adipose factor, enzymes of mitochondrial fatty acid oxidation). These changes paralleled inactivation of the retinoblastoma protein and were preceded by an early RA-induced phosphorylation of p38 mitogen-activated protein kinase. UCP1 expression was not induced. CONCLUSION: The results indicate that RA directly favors remodeling of mature 3T3-L1 adipocytes in culture toward increased oxidative metabolism.

AB - BACKGROUND/AIMS: In rodents, retinoic acid (RA) treatment favors loss of body fat mass and the acquisition of brown fat features in white fat depots. In this work, we sought to examine to what extent these RA effects are cell autonomous or dependent on systemic factors. METHODS: Parameters of lipid metabolism and related gene expression were analyzed in differentiated 3T3-L1 adipocytes after exposure to RA or vehicle. RESULTS: Treatment with RA resulted in decreased cellular triacylglycerol content and increased basal lipolysis and fatty acid oxidation rate. At the mRNA level, RA treatment led to a reduced expression of adipogenic/lipogenic transcription factors (peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, rexinoid receptor alpha) and two purported suppressors of lipolysis and oxidative metabolism (CIDEA and receptor-interacting protein 140), and to an increased expression of proteins favoring fat oxidation (peroxisome proliferator-activated receptor gamma coactivator-1alpha, uncoupling protein 2, fasting-induced adipose factor, enzymes of mitochondrial fatty acid oxidation). These changes paralleled inactivation of the retinoblastoma protein and were preceded by an early RA-induced phosphorylation of p38 mitogen-activated protein kinase. UCP1 expression was not induced. CONCLUSION: The results indicate that RA directly favors remodeling of mature 3T3-L1 adipocytes in culture toward increased oxidative metabolism.

KW - 3T3-L1 Cells

KW - Adipocytes

KW - Adipogenesis

KW - Animals

KW - Fatty Acids

KW - Gene Expression Regulation

KW - Ion Channels

KW - Lipid Metabolism

KW - Lipogenesis

KW - Mice

KW - Mitochondrial Proteins

KW - Oxidation-Reduction

KW - Phosphorylation

KW - Retinoblastoma Protein

KW - Transcription Factors

KW - Tretinoin

KW - Triglycerides

KW - p38 Mitogen-Activated Protein Kinases

U2 - 10.1159/0000110717

DO - 10.1159/0000110717

M3 - Journal article

C2 - 17975308

VL - 20

SP - 1061

EP - 1072

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 6

ER -

Mercader J, Madsen L, Felipe F, Palou A, Kristiansen K, Bonet ML. All-trans retinoic acid increases oxidative metabolism in mature adipocytes. Cellular Physiology and Biochemistry. 2007 jan 1;20(6):1061-1072. https://doi.org/10.1159/0000110717