TY - JOUR
T1 - Age-dependent plasticity of sex pheromone response in the moth, Agrotis ipsilon: combined effects of octopamine and juvenile hormone
AU - Jarriault, David
AU - Barrozo, Romina B
AU - de Carvalho Pinto, Carlos J
AU - Greiner, Birgit
AU - Dufour, Marie-Cécile
AU - Masante-Roca, Ingwild
AU - Gramsbergen, Jan B
AU - Anton, Sylvia
AU - Gadenne, Christophe
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Male moths use sex pheromones to find their mating partners. In the moth, Agrotis ipsilon, the behavioral response and the neuron sensitivity within the primary olfactory centre, the antennal lobe (AL), to sex pheromone increase with age and juvenile hormone (JH) biosynthesis. By manipulating the JH level, we previously showed that JH controls this age-dependent neuronal plasticity, and that its effects are slow (within 2 days). We hypothesized that the hormonal effect might be indirect, and one neuromodulator candidate, which might serve as a mediator, is octopamine (OA). Here, we studied the effects of OA and an OA receptor antagonist, mianserin, on behavioral and AL neuron responses of mature and immature males during stimulation with sex pheromone. Our results indicate that, although OA injections enhanced the behavioral pheromone response in mature males, OA had no significant effect on behavior in immature males. However, mianserin injections decreased the behavioral response in mature males. AL neuron sensitivity increased after OA treatment in immature males, and decreased after mianserin treatment in mature males. Determination of OA levels in ALs of immature and mature males did not reveal any difference. To study the possible interactive effects of JH and OA, the behavioral pheromone response was analyzed in JH-deprived mature males injected with OA, and in immature males injected with fenoxycarb, a JH agonist, and mianserin. Results show that both JH and OA are necessary to elicit a behavioral response of A. ipsilon males to sex pheromone.
AB - Male moths use sex pheromones to find their mating partners. In the moth, Agrotis ipsilon, the behavioral response and the neuron sensitivity within the primary olfactory centre, the antennal lobe (AL), to sex pheromone increase with age and juvenile hormone (JH) biosynthesis. By manipulating the JH level, we previously showed that JH controls this age-dependent neuronal plasticity, and that its effects are slow (within 2 days). We hypothesized that the hormonal effect might be indirect, and one neuromodulator candidate, which might serve as a mediator, is octopamine (OA). Here, we studied the effects of OA and an OA receptor antagonist, mianserin, on behavioral and AL neuron responses of mature and immature males during stimulation with sex pheromone. Our results indicate that, although OA injections enhanced the behavioral pheromone response in mature males, OA had no significant effect on behavior in immature males. However, mianserin injections decreased the behavioral response in mature males. AL neuron sensitivity increased after OA treatment in immature males, and decreased after mianserin treatment in mature males. Determination of OA levels in ALs of immature and mature males did not reveal any difference. To study the possible interactive effects of JH and OA, the behavioral pheromone response was analyzed in JH-deprived mature males injected with OA, and in immature males injected with fenoxycarb, a JH agonist, and mianserin. Results show that both JH and OA are necessary to elicit a behavioral response of A. ipsilon males to sex pheromone.
KW - Action Potentials
KW - Aging
KW - Animals
KW - Behavior, Animal
KW - Chromatography, High Pressure Liquid
KW - Juvenile Hormones
KW - Male
KW - Mianserin
KW - Microelectrodes
KW - Moths
KW - Motor Activity
KW - Neurons
KW - Octopamine
KW - Phenylcarbamates
KW - Physical Stimulation
KW - Receptors, Biogenic Amine
KW - Sensory Thresholds
KW - Sex Attractants
U2 - 10.1016/j.yhbeh.2009.04.005
DO - 10.1016/j.yhbeh.2009.04.005
M3 - Journal article
C2 - 19409391
SN - 0018-506X
VL - 56
SP - 185
EP - 191
JO - Hormones and Behavior
JF - Hormones and Behavior
IS - 1
ER -