Aerococcus urinae and Aerococcus sanguinicola: Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques

Derya Carkaci, Xiaohui C. Nielsen, Kurt Fuursted, Robert Skov, Ole Skovgaard, Emilio P. Trallero, Reto Lienhard, Jenny Åhman, Erika Matuschek, Gunnar Kahlmeter, Jens J. Christensen*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftLetterForskningpeer review

77 Downloads (Pure)

Resumé

Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.

OriginalsprogEngelsk
TidsskriftOpen Microbiology Journal
Vol/bind11
Sider (fra-til)160-166
ISSN1874-2858
DOI
StatusUdgivet - 2017

Fingeraftryk

Vancomycin
Rifampin

Citer dette

Carkaci, Derya ; Nielsen, Xiaohui C. ; Fuursted, Kurt ; Skov, Robert ; Skovgaard, Ole ; Trallero, Emilio P. ; Lienhard, Reto ; Åhman, Jenny ; Matuschek, Erika ; Kahlmeter, Gunnar ; Christensen, Jens J. / Aerococcus urinae and Aerococcus sanguinicola : Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques. I: Open Microbiology Journal. 2017 ; Bind 11. s. 160-166.
@article{03126216c8ed4373a29025a8d67333a1,
title = "Aerococcus urinae and Aerococcus sanguinicola: Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques",
abstract = "Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.",
keywords = "Aerococcus sanguinicola, Aerococcus urinae, Antimicrobial susceptibility testing, Broth microdilution, Disk diffusion, Etest, Urinary tract infections",
author = "Derya Carkaci and Nielsen, {Xiaohui C.} and Kurt Fuursted and Robert Skov and Ole Skovgaard and Trallero, {Emilio P.} and Reto Lienhard and Jenny {\AA}hman and Erika Matuschek and Gunnar Kahlmeter and Christensen, {Jens J.}",
year = "2017",
doi = "10.2174/1874285801711010160",
language = "English",
volume = "11",
pages = "160--166",
journal = "The Open Microbiology Journal",
issn = "1874-2858",
publisher = "Bentham Open",

}

Aerococcus urinae and Aerococcus sanguinicola : Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques. / Carkaci, Derya; Nielsen, Xiaohui C.; Fuursted, Kurt; Skov, Robert; Skovgaard, Ole; Trallero, Emilio P.; Lienhard, Reto; Åhman, Jenny; Matuschek, Erika; Kahlmeter, Gunnar; Christensen, Jens J.

I: Open Microbiology Journal, Bind 11, 2017, s. 160-166.

Publikation: Bidrag til tidsskriftLetterForskningpeer review

TY - JOUR

T1 - Aerococcus urinae and Aerococcus sanguinicola

T2 - Susceptibility testing of 120 isolates to six antimicrobial agents using disk diffusion (EUCAST), etest, and broth microdilution techniques

AU - Carkaci, Derya

AU - Nielsen, Xiaohui C.

AU - Fuursted, Kurt

AU - Skov, Robert

AU - Skovgaard, Ole

AU - Trallero, Emilio P.

AU - Lienhard, Reto

AU - Åhman, Jenny

AU - Matuschek, Erika

AU - Kahlmeter, Gunnar

AU - Christensen, Jens J.

PY - 2017

Y1 - 2017

N2 - Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.

AB - Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released. Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin. Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms. Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results. Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.

KW - Aerococcus sanguinicola

KW - Aerococcus urinae

KW - Antimicrobial susceptibility testing

KW - Broth microdilution

KW - Disk diffusion

KW - Etest

KW - Urinary tract infections

U2 - 10.2174/1874285801711010160

DO - 10.2174/1874285801711010160

M3 - Letter

C2 - 29151992

AN - SCOPUS:85032736718

VL - 11

SP - 160

EP - 166

JO - The Open Microbiology Journal

JF - The Open Microbiology Journal

SN - 1874-2858

ER -