Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction

Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen, Lars Lund, Ditte Caroline Andersen, Søren Paludan Sheikh*

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Abstract

Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as well as in recent Pase 1 trials in humans. However, some studies indicate that diabetes negatively affects the mesenchymal stem cell pool, implying that ASCs from T2DM patients could have impaired regenerative capacity. Here, we directly compared ASCs from age-matched diabetic Goto–Kakizaki (ASCGK) and non-diabetic wild type rats (ASCWT) with regard to their phenotypes, proteomes and ability to rescue ED in normal rats. Despite ASCGK exhibiting a slightly lower proliferation rate, ASCGK and ASCWT proteomes were more or less identical, and after injections to corpus cavernosum they were equally efficient in restoring erectile function in a rat ED model entailing bilateral nerve crush injury. Moreover, molecular analysis of the corpus cavernosum tissue revealed that both ASCGK and ASCWT treated rats had increased induction of genes involved in recovering endothelial function. Thus, our finding argues that T2DM does not appear to be a limiting factor for autologous adipose stem cell therapy when correcting for ED.
OriginalsprogEngelsk
Artikelnummer1692
TidsskriftInternational Journal of Molecular Sciences
Vol/bind23
Udgave nummer3
ISSN1661-6596
DOI
StatusUdgivet - 1. feb. 2022

Bibliografisk note

Funding Information:
Funding: Please add: This work was supported by the Faculty of Health Science, University of Southern Denmark. Odense Denmark, the Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense Denmark as well as the Innovation Foundation Denmark (#7051-00001A).

Funding Information:
This work was supported by the Faculty of Health Science, University of Southern Denmark. Odense Denmark, the Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense Denmark as well as the Innovation Foundation Denmark (#7051-00001A).

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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