Adipose derived mesenchymal stem cells – Their osteogenicity and osteoblast in vitro mineralization on titanium granule carriers

Morten Dahl, Susanne Syberg, Niklas Rye Jørgensen, Else Marie Pinholt

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

Purpose: Adipose derived mesenchymal stem cells (ADMSCs) may be osteogenic, may generate neoangiogenisis and may be progenitors for differentiated osteoblast mineralization. Titanium granules may be suitable as carriers for these cells. The aim was to demonstrate the osteogenic potential of ADMSCs and the effect of porous non-oxidized (Ti) and oxidized titanium (TiO 2) granules as carriers for ADMSCs mineralization in vitro. Materials and methods: ADMSCs were isolated, cultivated in osteoblast medium and evaluated for alkaline phosphatase (ALP) assay, RNA isolation, and ALP staining. Osteoblast in vitro mineralization cells without granules or seeded on Ti or TiO 2 granules were evaluated for Alizarin Red assay and RNA isolation for later gene expressing. Results: ADMSCs express osteoblastic lineage genes, CBFA-1 and stain strongly for ALP. Mineralization was significantly higher for cells seeded on TiO 2 than on Ti granules or pure cells. Expression of ALPL and RUNX2 was significantly higher for cells seeded on TiO 2 granules and expression of COL1α1 for pure cells was significantly higher than for cells seeded on granules. Conclusion: ADMSCs have osteogenic potential. Mineralization was significantly high when cells were seeded on TiO 2 granules. TiO 2 granules may be used as carriers for adipose derived mesenchymal osteoblastic cells from laboratory bench to the patient.

OriginalsprogEngelsk
TidsskriftJournal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery
Vol/bind41
Udgave nummer8
Sider (fra-til)e213-e220
Antal sider8
ISSN1010-5182
DOI
StatusUdgivet - dec. 2013

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