Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures

Christoph Salzlechner, Tabasom Haghighi, Isabella Huebscher, Anders Runge Walther, Sophie Schell, Alexander Gardner, Gerhard Undt, Ricardo M.P. da Silva, Cécile A. Dreiss, Kathleen Fan, Eileen Gentleman*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Minimally invasive surgical procedures aiming to repair damaged maxillofacial tissues are hampered by its small, complex structures and difficult surgical access. Indeed, while arthroscopic procedures that deliver regenerative materials and/or cells are common in articulating joints such as the knee, there are currently no treatments that surgically place cells, regenerative factors or materials into maxillofacial tissues to foster bone, cartilage or muscle repair. Here, hyaluronic acid (HA)-based hydrogels are developed, which are suitable for use in minimally invasive procedures, that can adhere to the surrounding tissue, and deliver cells and potentially drugs. By modifying HA with both methacrylate (MA) and 3,4-dihydroxyphenylalanine (Dopa) groups using a completely aqueous synthesis route, it is shown that MA-HA-Dopa hydrogels can be applied under aqueous conditions, gel quickly using a standard surgical light, and adhere to tissue. Moreover, upon oxidation of the Dopa, human marrow stromal cells attach to hydrogels and survive when encapsulated within them. These observations show that when incorporated into HA-based hydrogels, Dopa moieties can foster cell and tissue interactions, ensuring surgical placement and potentially enabling delivery/recruitment of regenerative cells. The findings suggest that MA-HA-Dopa hydrogels may find use in minimally invasive procedures to foster maxillofacial tissue repair.

OriginalsprogEngelsk
Artikelnummer1901134
TidsskriftAdvanced Healthcare Materials
ISSN2192-2640
DOI
StatusAccepteret/In press - 1. jan. 2020

Fingeraftryk

Tissue regeneration
Hydrogels
Hyaluronic acid
Dihydroxyphenylalanine
Adhesives
Hyaluronic Acid
Tissue
Methacrylates
Repair
Cartilage
Stromal Cells
Cell Communication
Muscle
Knee
Bone
Gels
Joints
Muscles
Oxidation
Pharmaceutical Preparations

Citer dette

Salzlechner, C., Haghighi, T., Huebscher, I., Walther, A. R., Schell, S., Gardner, A., ... Gentleman, E. (Accepteret/In press). Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures. Advanced Healthcare Materials, [1901134]. https://doi.org/10.1002/adhm.201901134
Salzlechner, Christoph ; Haghighi, Tabasom ; Huebscher, Isabella ; Walther, Anders Runge ; Schell, Sophie ; Gardner, Alexander ; Undt, Gerhard ; da Silva, Ricardo M.P. ; Dreiss, Cécile A. ; Fan, Kathleen ; Gentleman, Eileen. / Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures. I: Advanced Healthcare Materials. 2020.
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abstract = "Minimally invasive surgical procedures aiming to repair damaged maxillofacial tissues are hampered by its small, complex structures and difficult surgical access. Indeed, while arthroscopic procedures that deliver regenerative materials and/or cells are common in articulating joints such as the knee, there are currently no treatments that surgically place cells, regenerative factors or materials into maxillofacial tissues to foster bone, cartilage or muscle repair. Here, hyaluronic acid (HA)-based hydrogels are developed, which are suitable for use in minimally invasive procedures, that can adhere to the surrounding tissue, and deliver cells and potentially drugs. By modifying HA with both methacrylate (MA) and 3,4-dihydroxyphenylalanine (Dopa) groups using a completely aqueous synthesis route, it is shown that MA-HA-Dopa hydrogels can be applied under aqueous conditions, gel quickly using a standard surgical light, and adhere to tissue. Moreover, upon oxidation of the Dopa, human marrow stromal cells attach to hydrogels and survive when encapsulated within them. These observations show that when incorporated into HA-based hydrogels, Dopa moieties can foster cell and tissue interactions, ensuring surgical placement and potentially enabling delivery/recruitment of regenerative cells. The findings suggest that MA-HA-Dopa hydrogels may find use in minimally invasive procedures to foster maxillofacial tissue repair.",
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Salzlechner, C, Haghighi, T, Huebscher, I, Walther, AR, Schell, S, Gardner, A, Undt, G, da Silva, RMP, Dreiss, CA, Fan, K & Gentleman, E 2020, 'Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures', Advanced Healthcare Materials. https://doi.org/10.1002/adhm.201901134

Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures. / Salzlechner, Christoph; Haghighi, Tabasom; Huebscher, Isabella; Walther, Anders Runge; Schell, Sophie; Gardner, Alexander; Undt, Gerhard; da Silva, Ricardo M.P.; Dreiss, Cécile A.; Fan, Kathleen; Gentleman, Eileen.

I: Advanced Healthcare Materials, 01.01.2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Adhesive Hydrogels for Maxillofacial Tissue Regeneration Using Minimally Invasive Procedures

AU - Salzlechner, Christoph

AU - Haghighi, Tabasom

AU - Huebscher, Isabella

AU - Walther, Anders Runge

AU - Schell, Sophie

AU - Gardner, Alexander

AU - Undt, Gerhard

AU - da Silva, Ricardo M.P.

AU - Dreiss, Cécile A.

AU - Fan, Kathleen

AU - Gentleman, Eileen

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Minimally invasive surgical procedures aiming to repair damaged maxillofacial tissues are hampered by its small, complex structures and difficult surgical access. Indeed, while arthroscopic procedures that deliver regenerative materials and/or cells are common in articulating joints such as the knee, there are currently no treatments that surgically place cells, regenerative factors or materials into maxillofacial tissues to foster bone, cartilage or muscle repair. Here, hyaluronic acid (HA)-based hydrogels are developed, which are suitable for use in minimally invasive procedures, that can adhere to the surrounding tissue, and deliver cells and potentially drugs. By modifying HA with both methacrylate (MA) and 3,4-dihydroxyphenylalanine (Dopa) groups using a completely aqueous synthesis route, it is shown that MA-HA-Dopa hydrogels can be applied under aqueous conditions, gel quickly using a standard surgical light, and adhere to tissue. Moreover, upon oxidation of the Dopa, human marrow stromal cells attach to hydrogels and survive when encapsulated within them. These observations show that when incorporated into HA-based hydrogels, Dopa moieties can foster cell and tissue interactions, ensuring surgical placement and potentially enabling delivery/recruitment of regenerative cells. The findings suggest that MA-HA-Dopa hydrogels may find use in minimally invasive procedures to foster maxillofacial tissue repair.

AB - Minimally invasive surgical procedures aiming to repair damaged maxillofacial tissues are hampered by its small, complex structures and difficult surgical access. Indeed, while arthroscopic procedures that deliver regenerative materials and/or cells are common in articulating joints such as the knee, there are currently no treatments that surgically place cells, regenerative factors or materials into maxillofacial tissues to foster bone, cartilage or muscle repair. Here, hyaluronic acid (HA)-based hydrogels are developed, which are suitable for use in minimally invasive procedures, that can adhere to the surrounding tissue, and deliver cells and potentially drugs. By modifying HA with both methacrylate (MA) and 3,4-dihydroxyphenylalanine (Dopa) groups using a completely aqueous synthesis route, it is shown that MA-HA-Dopa hydrogels can be applied under aqueous conditions, gel quickly using a standard surgical light, and adhere to tissue. Moreover, upon oxidation of the Dopa, human marrow stromal cells attach to hydrogels and survive when encapsulated within them. These observations show that when incorporated into HA-based hydrogels, Dopa moieties can foster cell and tissue interactions, ensuring surgical placement and potentially enabling delivery/recruitment of regenerative cells. The findings suggest that MA-HA-Dopa hydrogels may find use in minimally invasive procedures to foster maxillofacial tissue repair.

KW - adhesivity

KW - biomaterials

KW - hydrogels

KW - minimally invasive surgery

KW - tissue engineering

U2 - 10.1002/adhm.201901134

DO - 10.1002/adhm.201901134

M3 - Journal article

C2 - 31943865

AN - SCOPUS:85077869056

JO - Advanced Healthcare Materials

JF - Advanced Healthcare Materials

SN - 2192-2640

M1 - 1901134

ER -