Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments

Stefano Bonaglia*, Elias Broman, Björn Brindefalk, Erika Hedlund, Tomas Hjorth, Carl Rolff, Francisco J.A. Nascimento, Klas Udekwu, Jonas S. Gunnarsson

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Biodegradation by microorganisms is a useful tool that helps alleviating hydrocarbon pollution in nature. Microbes are more efficient in degradation under aerobic than anaerobic conditions, but the majority of sediment by volume is generally anoxic. Incubation experiments were conducted to study the biodegradation potential of naphthalene—a common polycyclic aromatic hydrocarbon (PAH)—and the diversity of microbial communities in presence/absence of activated carbon (AC) under aerobic/anaerobic conditions. Radio-respirometry experiments with endogenous microorganisms indicated that degradation of naphthalene was strongly stimulated (96%) by the AC addition under anaerobic conditions. In aerobic conditions, however, AC had no effects on naphthalene biodegradation. Bioaugmentation tests with cultured microbial populations grown on naphthalene showed that AC further stimulated (92%) naphthalene degradation in anoxia. Analysis of the 16S rRNA gene sequences implied that sediment amendment with AC increased microbial community diversity and changed community structure. Moreover, the relative abundance of Geobacter, Thiobacillus, Sulfuricurvum, and methanogenic archaea increased sharply after amendment with AC under anaerobic conditions. These results may be explained by the fact that AC particles promoted direct interspecies electron transfer (DIET) between microorganisms involved in PAH degradation pathways. We suggest that important ecosystem functions mediated by microbes—such as hydrocarbon degradation—can be induced and that AC enrichment strategies can be exploited for facilitating bioremediation of anoxic oil-contaminated sediments and soils.

OriginalsprogEngelsk
Artikelnummer126023
TidsskriftChemosphere
Vol/bind248
ISSN0045-6535
DOI
StatusUdgivet - jun. 2020

Fingeraftryk

anoxic conditions
activated carbon
biodegradation
PAH
oil
naphthalene
sediment
degradation
microorganism
microbial community
hydrocarbon
anoxia
ecosystem function
oxic conditions
bioremediation
relative abundance
community structure
experiment
incubation
radio

Citer dette

Bonaglia, S., Broman, E., Brindefalk, B., Hedlund, E., Hjorth, T., Rolff, C., ... Gunnarsson, J. S. (2020). Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments. Chemosphere, 248, [126023]. https://doi.org/10.1016/j.chemosphere.2020.126023
Bonaglia, Stefano ; Broman, Elias ; Brindefalk, Björn ; Hedlund, Erika ; Hjorth, Tomas ; Rolff, Carl ; Nascimento, Francisco J.A. ; Udekwu, Klas ; Gunnarsson, Jonas S. / Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments. I: Chemosphere. 2020 ; Bind 248.
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title = "Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments",
abstract = "Biodegradation by microorganisms is a useful tool that helps alleviating hydrocarbon pollution in nature. Microbes are more efficient in degradation under aerobic than anaerobic conditions, but the majority of sediment by volume is generally anoxic. Incubation experiments were conducted to study the biodegradation potential of naphthalene—a common polycyclic aromatic hydrocarbon (PAH)—and the diversity of microbial communities in presence/absence of activated carbon (AC) under aerobic/anaerobic conditions. Radio-respirometry experiments with endogenous microorganisms indicated that degradation of naphthalene was strongly stimulated (96{\%}) by the AC addition under anaerobic conditions. In aerobic conditions, however, AC had no effects on naphthalene biodegradation. Bioaugmentation tests with cultured microbial populations grown on naphthalene showed that AC further stimulated (92{\%}) naphthalene degradation in anoxia. Analysis of the 16S rRNA gene sequences implied that sediment amendment with AC increased microbial community diversity and changed community structure. Moreover, the relative abundance of Geobacter, Thiobacillus, Sulfuricurvum, and methanogenic archaea increased sharply after amendment with AC under anaerobic conditions. These results may be explained by the fact that AC particles promoted direct interspecies electron transfer (DIET) between microorganisms involved in PAH degradation pathways. We suggest that important ecosystem functions mediated by microbes—such as hydrocarbon degradation—can be induced and that AC enrichment strategies can be exploited for facilitating bioremediation of anoxic oil-contaminated sediments and soils.",
keywords = "16S rRNA sequencing, Bioremediation, Hydrocarbon pollution, Microbial communities, Naphthalene, Powdered activated carbon (PAC)",
author = "Stefano Bonaglia and Elias Broman and Bj{\"o}rn Brindefalk and Erika Hedlund and Tomas Hjorth and Carl Rolff and Nascimento, {Francisco J.A.} and Klas Udekwu and Gunnarsson, {Jonas S.}",
year = "2020",
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doi = "10.1016/j.chemosphere.2020.126023",
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Bonaglia, S, Broman, E, Brindefalk, B, Hedlund, E, Hjorth, T, Rolff, C, Nascimento, FJA, Udekwu, K & Gunnarsson, JS 2020, 'Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments', Chemosphere, bind 248, 126023. https://doi.org/10.1016/j.chemosphere.2020.126023

Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments. / Bonaglia, Stefano; Broman, Elias; Brindefalk, Björn; Hedlund, Erika; Hjorth, Tomas; Rolff, Carl; Nascimento, Francisco J.A.; Udekwu, Klas; Gunnarsson, Jonas S.

I: Chemosphere, Bind 248, 126023, 06.2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Activated carbon stimulates microbial diversity and PAH biodegradation under anaerobic conditions in oil-polluted sediments

AU - Bonaglia, Stefano

AU - Broman, Elias

AU - Brindefalk, Björn

AU - Hedlund, Erika

AU - Hjorth, Tomas

AU - Rolff, Carl

AU - Nascimento, Francisco J.A.

AU - Udekwu, Klas

AU - Gunnarsson, Jonas S.

PY - 2020/6

Y1 - 2020/6

N2 - Biodegradation by microorganisms is a useful tool that helps alleviating hydrocarbon pollution in nature. Microbes are more efficient in degradation under aerobic than anaerobic conditions, but the majority of sediment by volume is generally anoxic. Incubation experiments were conducted to study the biodegradation potential of naphthalene—a common polycyclic aromatic hydrocarbon (PAH)—and the diversity of microbial communities in presence/absence of activated carbon (AC) under aerobic/anaerobic conditions. Radio-respirometry experiments with endogenous microorganisms indicated that degradation of naphthalene was strongly stimulated (96%) by the AC addition under anaerobic conditions. In aerobic conditions, however, AC had no effects on naphthalene biodegradation. Bioaugmentation tests with cultured microbial populations grown on naphthalene showed that AC further stimulated (92%) naphthalene degradation in anoxia. Analysis of the 16S rRNA gene sequences implied that sediment amendment with AC increased microbial community diversity and changed community structure. Moreover, the relative abundance of Geobacter, Thiobacillus, Sulfuricurvum, and methanogenic archaea increased sharply after amendment with AC under anaerobic conditions. These results may be explained by the fact that AC particles promoted direct interspecies electron transfer (DIET) between microorganisms involved in PAH degradation pathways. We suggest that important ecosystem functions mediated by microbes—such as hydrocarbon degradation—can be induced and that AC enrichment strategies can be exploited for facilitating bioremediation of anoxic oil-contaminated sediments and soils.

AB - Biodegradation by microorganisms is a useful tool that helps alleviating hydrocarbon pollution in nature. Microbes are more efficient in degradation under aerobic than anaerobic conditions, but the majority of sediment by volume is generally anoxic. Incubation experiments were conducted to study the biodegradation potential of naphthalene—a common polycyclic aromatic hydrocarbon (PAH)—and the diversity of microbial communities in presence/absence of activated carbon (AC) under aerobic/anaerobic conditions. Radio-respirometry experiments with endogenous microorganisms indicated that degradation of naphthalene was strongly stimulated (96%) by the AC addition under anaerobic conditions. In aerobic conditions, however, AC had no effects on naphthalene biodegradation. Bioaugmentation tests with cultured microbial populations grown on naphthalene showed that AC further stimulated (92%) naphthalene degradation in anoxia. Analysis of the 16S rRNA gene sequences implied that sediment amendment with AC increased microbial community diversity and changed community structure. Moreover, the relative abundance of Geobacter, Thiobacillus, Sulfuricurvum, and methanogenic archaea increased sharply after amendment with AC under anaerobic conditions. These results may be explained by the fact that AC particles promoted direct interspecies electron transfer (DIET) between microorganisms involved in PAH degradation pathways. We suggest that important ecosystem functions mediated by microbes—such as hydrocarbon degradation—can be induced and that AC enrichment strategies can be exploited for facilitating bioremediation of anoxic oil-contaminated sediments and soils.

KW - 16S rRNA sequencing

KW - Bioremediation

KW - Hydrocarbon pollution

KW - Microbial communities

KW - Naphthalene

KW - Powdered activated carbon (PAC)

UR - http://www.scopus.com/inward/record.url?scp=85078415486&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2020.126023

DO - 10.1016/j.chemosphere.2020.126023

M3 - Journal article

C2 - 32007777

AN - SCOPUS:85078415486

VL - 248

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

M1 - 126023

ER -