Accumulation and detoxication responses of the gastropod Lymnaea stagnalis to single and combined exposures to natural (cyanobacteria) and anthropogenic (the herbicide RoundUp® Flash) stressors

Emilie Lance, J. Desprat, B. F. Holbech, Claudia Gerard, Myriam Bormans, L. A. Lawton, Cathryn Edwards, C. Wiegand

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Freshwater gastropods are increasingly exposed to multiple stressors in the field such as the herbicide glyphosate in Roundup formulations and cyanobacterial blooms either producing or not producing microcystins (MCs), potentially leading to interacting effects. Here, the responses of Lymnaea stagnalis to a 21-day exposure to non-MC or MC-producing (33 μg L−1) Planktothrix agardhii alone or in combination with the commercial formulation RoundUp® Flash at a concentration of 1 μg L−1 glyphosate, followed by 14 days of depuration, were studied via i) accumulation of free and bound MCs in tissues, and ii) activities of anti-oxidant (catalase CAT) and biotransformation (glutathione-S-transferase GST) enzymes. During the intoxication, the cyanobacterial exposure induced an early increase of CAT activity, independently of the MC content, probably related to the production of secondary cyanobacterial metabolites. The GST activity was induced by RoundUp® Flash alone or in combination with non MC-producing cyanobacteria, but was inhibited by MC-producing cyanobacteria with or without RoundUp® Flash. Moreover, MC accumulation in L. stagnalis was 3.2 times increased when snails were concomitantly exposed to MC-producing cyanobacteria with RoundUp®, suggesting interacting effects of MCs on biotransformation processes. The potent inhibition of detoxication systems by MCs and RoundUp® Flash was reversible during the depuration, during which CAT and GST activities were significantly higher in snails previously exposed to MC-producing cyanobacteria with or without RoundUp® Flash than in other conditions, probably related to the oxidative stress caused by accumulated MCs remaining in tissues. © 2016 Elsevier B.V.
OriginalsprogEngelsk
TidsskriftAquatic Toxicology
Vol/bind177
Sider (fra-til)116-124
ISSN0166-445X
DOI
StatusUdgivet - 2016

Citer dette

@article{ba3f6973a9dc43388eba13c044502a55,
title = "Accumulation and detoxication responses of the gastropod Lymnaea stagnalis to single and combined exposures to natural (cyanobacteria) and anthropogenic (the herbicide RoundUp{\circledR} Flash) stressors",
abstract = "Freshwater gastropods are increasingly exposed to multiple stressors in the field such as the herbicide glyphosate in Roundup formulations and cyanobacterial blooms either producing or not producing microcystins (MCs), potentially leading to interacting effects. Here, the responses of Lymnaea stagnalis to a 21-day exposure to non-MC or MC-producing (33 μg L−1) Planktothrix agardhii alone or in combination with the commercial formulation RoundUp{\circledR} Flash at a concentration of 1 μg L−1 glyphosate, followed by 14 days of depuration, were studied via i) accumulation of free and bound MCs in tissues, and ii) activities of anti-oxidant (catalase CAT) and biotransformation (glutathione-S-transferase GST) enzymes. During the intoxication, the cyanobacterial exposure induced an early increase of CAT activity, independently of the MC content, probably related to the production of secondary cyanobacterial metabolites. The GST activity was induced by RoundUp{\circledR} Flash alone or in combination with non MC-producing cyanobacteria, but was inhibited by MC-producing cyanobacteria with or without RoundUp{\circledR} Flash. Moreover, MC accumulation in L. stagnalis was 3.2 times increased when snails were concomitantly exposed to MC-producing cyanobacteria with RoundUp{\circledR}, suggesting interacting effects of MCs on biotransformation processes. The potent inhibition of detoxication systems by MCs and RoundUp{\circledR} Flash was reversible during the depuration, during which CAT and GST activities were significantly higher in snails previously exposed to MC-producing cyanobacteria with or without RoundUp{\circledR} Flash than in other conditions, probably related to the oxidative stress caused by accumulated MCs remaining in tissues. {\circledC} 2016 Elsevier B.V.",
author = "Emilie Lance and J. Desprat and Holbech, {B. F.} and Claudia Gerard and Myriam Bormans and Lawton, {L. A.} and Cathryn Edwards and C. Wiegand",
year = "2016",
doi = "10.1016/j.aquatox.2016.05.024",
language = "English",
volume = "177",
pages = "116--124",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",

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Accumulation and detoxication responses of the gastropod Lymnaea stagnalis to single and combined exposures to natural (cyanobacteria) and anthropogenic (the herbicide RoundUp® Flash) stressors. / Lance, Emilie; Desprat, J.; Holbech, B. F.; Gerard, Claudia; Bormans, Myriam; Lawton, L. A.; Edwards, Cathryn; Wiegand, C.

I: Aquatic Toxicology, Bind 177, 2016, s. 116-124.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Accumulation and detoxication responses of the gastropod Lymnaea stagnalis to single and combined exposures to natural (cyanobacteria) and anthropogenic (the herbicide RoundUp® Flash) stressors

AU - Lance, Emilie

AU - Desprat, J.

AU - Holbech, B. F.

AU - Gerard, Claudia

AU - Bormans, Myriam

AU - Lawton, L. A.

AU - Edwards, Cathryn

AU - Wiegand, C.

PY - 2016

Y1 - 2016

N2 - Freshwater gastropods are increasingly exposed to multiple stressors in the field such as the herbicide glyphosate in Roundup formulations and cyanobacterial blooms either producing or not producing microcystins (MCs), potentially leading to interacting effects. Here, the responses of Lymnaea stagnalis to a 21-day exposure to non-MC or MC-producing (33 μg L−1) Planktothrix agardhii alone or in combination with the commercial formulation RoundUp® Flash at a concentration of 1 μg L−1 glyphosate, followed by 14 days of depuration, were studied via i) accumulation of free and bound MCs in tissues, and ii) activities of anti-oxidant (catalase CAT) and biotransformation (glutathione-S-transferase GST) enzymes. During the intoxication, the cyanobacterial exposure induced an early increase of CAT activity, independently of the MC content, probably related to the production of secondary cyanobacterial metabolites. The GST activity was induced by RoundUp® Flash alone or in combination with non MC-producing cyanobacteria, but was inhibited by MC-producing cyanobacteria with or without RoundUp® Flash. Moreover, MC accumulation in L. stagnalis was 3.2 times increased when snails were concomitantly exposed to MC-producing cyanobacteria with RoundUp®, suggesting interacting effects of MCs on biotransformation processes. The potent inhibition of detoxication systems by MCs and RoundUp® Flash was reversible during the depuration, during which CAT and GST activities were significantly higher in snails previously exposed to MC-producing cyanobacteria with or without RoundUp® Flash than in other conditions, probably related to the oxidative stress caused by accumulated MCs remaining in tissues. © 2016 Elsevier B.V.

AB - Freshwater gastropods are increasingly exposed to multiple stressors in the field such as the herbicide glyphosate in Roundup formulations and cyanobacterial blooms either producing or not producing microcystins (MCs), potentially leading to interacting effects. Here, the responses of Lymnaea stagnalis to a 21-day exposure to non-MC or MC-producing (33 μg L−1) Planktothrix agardhii alone or in combination with the commercial formulation RoundUp® Flash at a concentration of 1 μg L−1 glyphosate, followed by 14 days of depuration, were studied via i) accumulation of free and bound MCs in tissues, and ii) activities of anti-oxidant (catalase CAT) and biotransformation (glutathione-S-transferase GST) enzymes. During the intoxication, the cyanobacterial exposure induced an early increase of CAT activity, independently of the MC content, probably related to the production of secondary cyanobacterial metabolites. The GST activity was induced by RoundUp® Flash alone or in combination with non MC-producing cyanobacteria, but was inhibited by MC-producing cyanobacteria with or without RoundUp® Flash. Moreover, MC accumulation in L. stagnalis was 3.2 times increased when snails were concomitantly exposed to MC-producing cyanobacteria with RoundUp®, suggesting interacting effects of MCs on biotransformation processes. The potent inhibition of detoxication systems by MCs and RoundUp® Flash was reversible during the depuration, during which CAT and GST activities were significantly higher in snails previously exposed to MC-producing cyanobacteria with or without RoundUp® Flash than in other conditions, probably related to the oxidative stress caused by accumulated MCs remaining in tissues. © 2016 Elsevier B.V.

U2 - 10.1016/j.aquatox.2016.05.024

DO - 10.1016/j.aquatox.2016.05.024

M3 - Journal article

VL - 177

SP - 116

EP - 124

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

ER -