ACBP - a PPAR and SREBP modulated housekeeping gene

Ditte Neess, Pia Kiilerich, Maria B Sandberg, Torben Helledie, Ronni Nielsen, Susanne Mandrup

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2006-Mar
OriginalsprogEngelsk
TidsskriftMolecular and Cellular Biochemistry
Vol/bind284
Udgave nummer1-2
Sider (fra-til)149-157
Antal sider8
ISSN0300-8177
DOI
StatusUdgivet - 1. mar. 2006

Fingeraftryk

Diazepam Binding Inhibitor
Peroxisome Proliferator-Activated Receptors
Essential Genes
Genes
PPAR alpha
Sterol Regulatory Element Binding Protein 1
PPAR gamma
Adipocytes
Transcriptional Activation
Fasting
Peroxisome Proliferators
Acyl Coenzyme A
Transcription
Fibroblasts
Introns
Rats
Hepatocytes
Hepatocellular Carcinoma
Carrier Proteins
Esters

Citer dette

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title = "ACBP - a PPAR and SREBP modulated housekeeping gene",
abstract = "The acyl-CoA binding protein (ACBP) is a 10 kD intracellular lipid binding protein that binds and transports acyl-CoA esters. The protein is expressed in most cell types at low levels; however, expression differs markedly between different cell types with expression being particularly high in e.g. cells with a high turnover of fatty acids. We show here that the relatively high basal promoter activity of the rat ACBP gene in fibroblasts and hepatoma cells relies on sequences between -331 to -182 and on the Sp1 and NF-Y sites at -172 and -143, respectively. The basal transcription is modulated by members of the PPAR and SREBP families. In adipocytes, PPARgamma is in part responsible for the induction during adipocyte differentiation, but other transcription factors appear to play a role as well. In hepatocytes, SREBP-1c is the main regulator of ACBP in response to changes in insulin levels during fasting/refeeding. PPARalpha counteracts this effect by stimulating ACBP expression during fasting. In addition, PPARalpha mediates the induction of ACBP expression in response to peroxisome proliferators. PPARalpha and PPARgamma do not require sequences upstream of -182 for transactivation; however, SREBP-1c requires the synergistic action of sequences in intron 1 for transactivation of the ACBP promoter.",
keywords = "Adipocytes, Adipogenesis, Animals, Base Sequence, Cell Differentiation, Cells, Cultured, Diazepam Binding Inhibitor, Fibroblasts, Gene Expression Regulation, Hepatocytes, Insulin, Introns, Mice, Mice, Inbred C57BL, Molecular Sequence Data, PPAR alpha, PPAR gamma, Peroxisome Proliferators, Promoter Regions (Genetics), Rats, Sterol Regulatory Element Binding Protein 1, Transcription, Genetic",
author = "Ditte Neess and Pia Kiilerich and Sandberg, {Maria B} and Torben Helledie and Ronni Nielsen and Susanne Mandrup",
year = "2006",
month = "3",
day = "1",
doi = "10.1007/s11010-005-9039-9",
language = "English",
volume = "284",
pages = "149--157",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer",
number = "1-2",

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ACBP - a PPAR and SREBP modulated housekeeping gene. / Neess, Ditte; Kiilerich, Pia; Sandberg, Maria B; Helledie, Torben; Nielsen, Ronni; Mandrup, Susanne.

I: Molecular and Cellular Biochemistry, Bind 284, Nr. 1-2, 01.03.2006, s. 149-157.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - ACBP - a PPAR and SREBP modulated housekeeping gene

AU - Neess, Ditte

AU - Kiilerich, Pia

AU - Sandberg, Maria B

AU - Helledie, Torben

AU - Nielsen, Ronni

AU - Mandrup, Susanne

PY - 2006/3/1

Y1 - 2006/3/1

N2 - The acyl-CoA binding protein (ACBP) is a 10 kD intracellular lipid binding protein that binds and transports acyl-CoA esters. The protein is expressed in most cell types at low levels; however, expression differs markedly between different cell types with expression being particularly high in e.g. cells with a high turnover of fatty acids. We show here that the relatively high basal promoter activity of the rat ACBP gene in fibroblasts and hepatoma cells relies on sequences between -331 to -182 and on the Sp1 and NF-Y sites at -172 and -143, respectively. The basal transcription is modulated by members of the PPAR and SREBP families. In adipocytes, PPARgamma is in part responsible for the induction during adipocyte differentiation, but other transcription factors appear to play a role as well. In hepatocytes, SREBP-1c is the main regulator of ACBP in response to changes in insulin levels during fasting/refeeding. PPARalpha counteracts this effect by stimulating ACBP expression during fasting. In addition, PPARalpha mediates the induction of ACBP expression in response to peroxisome proliferators. PPARalpha and PPARgamma do not require sequences upstream of -182 for transactivation; however, SREBP-1c requires the synergistic action of sequences in intron 1 for transactivation of the ACBP promoter.

AB - The acyl-CoA binding protein (ACBP) is a 10 kD intracellular lipid binding protein that binds and transports acyl-CoA esters. The protein is expressed in most cell types at low levels; however, expression differs markedly between different cell types with expression being particularly high in e.g. cells with a high turnover of fatty acids. We show here that the relatively high basal promoter activity of the rat ACBP gene in fibroblasts and hepatoma cells relies on sequences between -331 to -182 and on the Sp1 and NF-Y sites at -172 and -143, respectively. The basal transcription is modulated by members of the PPAR and SREBP families. In adipocytes, PPARgamma is in part responsible for the induction during adipocyte differentiation, but other transcription factors appear to play a role as well. In hepatocytes, SREBP-1c is the main regulator of ACBP in response to changes in insulin levels during fasting/refeeding. PPARalpha counteracts this effect by stimulating ACBP expression during fasting. In addition, PPARalpha mediates the induction of ACBP expression in response to peroxisome proliferators. PPARalpha and PPARgamma do not require sequences upstream of -182 for transactivation; however, SREBP-1c requires the synergistic action of sequences in intron 1 for transactivation of the ACBP promoter.

KW - Adipocytes

KW - Adipogenesis

KW - Animals

KW - Base Sequence

KW - Cell Differentiation

KW - Cells, Cultured

KW - Diazepam Binding Inhibitor

KW - Fibroblasts

KW - Gene Expression Regulation

KW - Hepatocytes

KW - Insulin

KW - Introns

KW - Mice

KW - Mice, Inbred C57BL

KW - Molecular Sequence Data

KW - PPAR alpha

KW - PPAR gamma

KW - Peroxisome Proliferators

KW - Promoter Regions (Genetics)

KW - Rats

KW - Sterol Regulatory Element Binding Protein 1

KW - Transcription, Genetic

U2 - 10.1007/s11010-005-9039-9

DO - 10.1007/s11010-005-9039-9

M3 - Journal article

C2 - 16411019

VL - 284

SP - 149

EP - 157

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -