Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

Olga A. Mirgorodskaya, Roman Körner*, Yuri P. Kozmin, Peter Roepstorff

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Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

Resumé

Amino acid analysis is among the most accurate methods for absolute quantification of proteins and peptides. Here we combine acid hydrolysis with the addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10 fmol of protein standards with errors below 10% has been demonstrated using this method.

OriginalsprogEngelsk
TitelAmino Acid Analysis : Methods and Protocols
Antal sider6
Vol/bind2030
ForlagHumana Press
Publikationsdato2019
Sider11-16
Kapitel2
ISBN (Trykt)978-1-4939-9638-4
ISBN (Elektronisk)978-1-4939-9639-1
DOI
StatusUdgivet - 2019
NavnMethods in Molecular Biology
Vol/bind2030
ISSN1064-3745

Fingeraftryk

Hydrolysis
Amino Acids
Acids
Proteins
Peptides

Citer dette

Mirgorodskaya, O. A., Körner, R., Kozmin, Y. P., & Roepstorff, P. (2019). Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. I Amino Acid Analysis: Methods and Protocols (Bind 2030, s. 11-16). Humana Press. Methods in Molecular Biology, Bind. 2030 https://doi.org/10.1007/978-1-4939-9639-1_2
Mirgorodskaya, Olga A. ; Körner, Roman ; Kozmin, Yuri P. ; Roepstorff, Peter. / Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. Amino Acid Analysis: Methods and Protocols. Bind 2030 Humana Press, 2019. s. 11-16 (Methods in Molecular Biology, Bind 2030).
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Mirgorodskaya, OA, Körner, R, Kozmin, YP & Roepstorff, P 2019, Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. i Amino Acid Analysis: Methods and Protocols. bind 2030, Humana Press, Methods in Molecular Biology, bind 2030, s. 11-16. https://doi.org/10.1007/978-1-4939-9639-1_2

Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. / Mirgorodskaya, Olga A.; Körner, Roman; Kozmin, Yuri P.; Roepstorff, Peter.

Amino Acid Analysis: Methods and Protocols. Bind 2030 Humana Press, 2019. s. 11-16 (Methods in Molecular Biology, Bind 2030).

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

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AU - Kozmin, Yuri P.

AU - Roepstorff, Peter

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KW - Isotope-labeled amino acids

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Mirgorodskaya OA, Körner R, Kozmin YP, Roepstorff P. Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. I Amino Acid Analysis: Methods and Protocols. Bind 2030. Humana Press. 2019. s. 11-16. (Methods in Molecular Biology, Bind 2030). https://doi.org/10.1007/978-1-4939-9639-1_2