A simplification of the enzyme-linked immunospot technique. Increased sensitivity for cells secreting IgG antibodies to Haemophilus influenzae type b capsular polysaccharide

T Barington, S Sparholt, L Juul, C Heilmann

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1992-Dec-8
OriginalsprogEngelsk
TidsskriftJournal of Immunological Methods
Vol/bind156
Udgave nummer2
Sider (fra-til)191-8
Antal sider7
ISSN0022-1759
StatusUdgivet - 8. dec. 1992

Fingeraftryk

Haemophilus influenzae type b
Enzymes

Citer dette

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title = "A simplification of the enzyme-linked immunospot technique. Increased sensitivity for cells secreting IgG antibodies to Haemophilus influenzae type b capsular polysaccharide",
abstract = "A simplified enzyme-linked immunospot (ELISPOT) technique is described for the detection of cells secreting antibodies to tetanus toxoid (TT), diphtheria toxoid (DT) or Haemophilus influenzae type b capsular polysaccharide (PRP). By combining the cell suspension with the enzyme-linked secondary antibodies in one incubation, the second incubation and washing procedure could be omitted from the original technique. The simplified assay had the same sensitivity for anti-TT and anti-DT spot-forming cells as the ordinary ELISPOT assay. The IgG anti-PRP spots were, however, improved both in quality and in quantity (median: 40{\%} more spots), while the detection of IgM and IgA anti-PRP spot-forming cells was the same in the two techniques. This simplified technique can probably also be used to save time in other antigen systems and should be considered when designing ELISPOT assays for the detection of polysaccharide-specific antibody-secreting cells.",
keywords = "Antibodies, Bacterial, Antigens, Bacterial, Bacterial Capsules, Diphtheria Toxoid, Haemophilus influenzae, Humans, Immunoenzyme Techniques, Immunoglobulin Isotypes, Polysaccharides, Bacterial, Tetanus Toxoid",
author = "T Barington and S Sparholt and L Juul and C Heilmann",
year = "1992",
month = "12",
day = "8",
language = "English",
volume = "156",
pages = "191--8",
journal = "Journal of Immunological Methods",
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A simplification of the enzyme-linked immunospot technique. Increased sensitivity for cells secreting IgG antibodies to Haemophilus influenzae type b capsular polysaccharide. / Barington, T; Sparholt, S; Juul, L; Heilmann, C.

I: Journal of Immunological Methods, Bind 156, Nr. 2, 08.12.1992, s. 191-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A simplification of the enzyme-linked immunospot technique. Increased sensitivity for cells secreting IgG antibodies to Haemophilus influenzae type b capsular polysaccharide

AU - Barington, T

AU - Sparholt, S

AU - Juul, L

AU - Heilmann, C

PY - 1992/12/8

Y1 - 1992/12/8

N2 - A simplified enzyme-linked immunospot (ELISPOT) technique is described for the detection of cells secreting antibodies to tetanus toxoid (TT), diphtheria toxoid (DT) or Haemophilus influenzae type b capsular polysaccharide (PRP). By combining the cell suspension with the enzyme-linked secondary antibodies in one incubation, the second incubation and washing procedure could be omitted from the original technique. The simplified assay had the same sensitivity for anti-TT and anti-DT spot-forming cells as the ordinary ELISPOT assay. The IgG anti-PRP spots were, however, improved both in quality and in quantity (median: 40% more spots), while the detection of IgM and IgA anti-PRP spot-forming cells was the same in the two techniques. This simplified technique can probably also be used to save time in other antigen systems and should be considered when designing ELISPOT assays for the detection of polysaccharide-specific antibody-secreting cells.

AB - A simplified enzyme-linked immunospot (ELISPOT) technique is described for the detection of cells secreting antibodies to tetanus toxoid (TT), diphtheria toxoid (DT) or Haemophilus influenzae type b capsular polysaccharide (PRP). By combining the cell suspension with the enzyme-linked secondary antibodies in one incubation, the second incubation and washing procedure could be omitted from the original technique. The simplified assay had the same sensitivity for anti-TT and anti-DT spot-forming cells as the ordinary ELISPOT assay. The IgG anti-PRP spots were, however, improved both in quality and in quantity (median: 40% more spots), while the detection of IgM and IgA anti-PRP spot-forming cells was the same in the two techniques. This simplified technique can probably also be used to save time in other antigen systems and should be considered when designing ELISPOT assays for the detection of polysaccharide-specific antibody-secreting cells.

KW - Antibodies, Bacterial

KW - Antigens, Bacterial

KW - Bacterial Capsules

KW - Diphtheria Toxoid

KW - Haemophilus influenzae

KW - Humans

KW - Immunoenzyme Techniques

KW - Immunoglobulin Isotypes

KW - Polysaccharides, Bacterial

KW - Tetanus Toxoid

M3 - Journal article

VL - 156

SP - 191

EP - 198

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -