A reference preparation of creatine kinase BB isoenzyme

M. Mathieu*, J. P. Steghens, M. Horder, D. W. Moss, E. Colinet, C. Profilis

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Resumé

Creatine kinase (CK; EC 2.7.3.2) catalytic activity in serum is widely measured in clinical chemistry practice and provides information for diagnosis and follow-up in many pathological conditions affecting heart, muscle, and brain. Depending on the organ involved, the predominant CK isoenzyme in serum varies. However, routine methods measure total CK catalytic activity, and standardized methods for doing so have been recommended by the International Federation of Clinical Chemistry and by several national scientific societies. Many commercial kits for those methods are now available. With use of a reference material for CK, commercial reagents can be compared with standardized methods, improving confidence in the results. Here we present a reference preparation of CK consisting of the BB isoenzyme purified from human placentae. We describe the procedure of purification and the properties of the lyophilized preparation of CK-BB, which has been certified by the Community Bureau of Reference of the Commission of the European Communities under the designation CRM 299. The preparation can be used to calibrate assays of the catalytic activity of CK- MM and CK-MB, as well as CK-BB.

OriginalsprogEngelsk
TidsskriftClinical Chemistry
Vol/bind39
Udgave nummer9
Sider (fra-til)1894-1898
Antal sider5
ISSN0009-9147
StatusUdgivet - 1. jan. 1993

Fingeraftryk

BB Form Creatine Kinase
Isoenzymes
Catalyst activity
Clinical Chemistry
Creatine Kinase
Purification
Muscle
Assays
Brain
European Union
Serum
Placenta
Myocardium

Citer dette

Mathieu, M., Steghens, J. P., Horder, M., Moss, D. W., Colinet, E., & Profilis, C. (1993). A reference preparation of creatine kinase BB isoenzyme. Clinical Chemistry, 39(9), 1894-1898.
Mathieu, M. ; Steghens, J. P. ; Horder, M. ; Moss, D. W. ; Colinet, E. ; Profilis, C. / A reference preparation of creatine kinase BB isoenzyme. I: Clinical Chemistry. 1993 ; Bind 39, Nr. 9. s. 1894-1898.
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Mathieu, M, Steghens, JP, Horder, M, Moss, DW, Colinet, E & Profilis, C 1993, 'A reference preparation of creatine kinase BB isoenzyme', Clinical Chemistry, bind 39, nr. 9, s. 1894-1898.

A reference preparation of creatine kinase BB isoenzyme. / Mathieu, M.; Steghens, J. P.; Horder, M.; Moss, D. W.; Colinet, E.; Profilis, C.

I: Clinical Chemistry, Bind 39, Nr. 9, 01.01.1993, s. 1894-1898.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A reference preparation of creatine kinase BB isoenzyme

AU - Mathieu, M.

AU - Steghens, J. P.

AU - Horder, M.

AU - Moss, D. W.

AU - Colinet, E.

AU - Profilis, C.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Creatine kinase (CK; EC 2.7.3.2) catalytic activity in serum is widely measured in clinical chemistry practice and provides information for diagnosis and follow-up in many pathological conditions affecting heart, muscle, and brain. Depending on the organ involved, the predominant CK isoenzyme in serum varies. However, routine methods measure total CK catalytic activity, and standardized methods for doing so have been recommended by the International Federation of Clinical Chemistry and by several national scientific societies. Many commercial kits for those methods are now available. With use of a reference material for CK, commercial reagents can be compared with standardized methods, improving confidence in the results. Here we present a reference preparation of CK consisting of the BB isoenzyme purified from human placentae. We describe the procedure of purification and the properties of the lyophilized preparation of CK-BB, which has been certified by the Community Bureau of Reference of the Commission of the European Communities under the designation CRM 299. The preparation can be used to calibrate assays of the catalytic activity of CK- MM and CK-MB, as well as CK-BB.

AB - Creatine kinase (CK; EC 2.7.3.2) catalytic activity in serum is widely measured in clinical chemistry practice and provides information for diagnosis and follow-up in many pathological conditions affecting heart, muscle, and brain. Depending on the organ involved, the predominant CK isoenzyme in serum varies. However, routine methods measure total CK catalytic activity, and standardized methods for doing so have been recommended by the International Federation of Clinical Chemistry and by several national scientific societies. Many commercial kits for those methods are now available. With use of a reference material for CK, commercial reagents can be compared with standardized methods, improving confidence in the results. Here we present a reference preparation of CK consisting of the BB isoenzyme purified from human placentae. We describe the procedure of purification and the properties of the lyophilized preparation of CK-BB, which has been certified by the Community Bureau of Reference of the Commission of the European Communities under the designation CRM 299. The preparation can be used to calibrate assays of the catalytic activity of CK- MM and CK-MB, as well as CK-BB.

KW - calibration

KW - enzyme activity

KW - reference material

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M3 - Journal article

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JO - Clinical Chemistry

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Mathieu M, Steghens JP, Horder M, Moss DW, Colinet E, Profilis C. A reference preparation of creatine kinase BB isoenzyme. Clinical Chemistry. 1993 jan 1;39(9):1894-1898.