A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Kasper Kannegård Karlsen, Anders Hauge Okholm, Jørgen Kjems, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry
Vol/bind21
Udgave nummer20
Sider (fra-til)6186-6190
Antal sider5
ISSN0968-0896
DOI
StatusUdgivet - 1. maj 2013

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