@article{37cd471048ea11ddb1a1000ea68e967b,
title = "A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes.",
abstract = "We have undertaken a systematic proteomic approach to purify and identify secreted factors that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional gel electrophoresis combined with nanoelectrospray tandem mass spectrometry, proteins that were specifically secreted by 3T3-L1 preadipocytes or adipocytes were identified. In addition to a number of previously reported molecules that are up- or down-regulated during this differentiation process (adipsin, adipocyte complement-related protein 30 kDa, complement C3, and fibronectin), we identified four secreted molecules that have not been shown previously to be expressed differentially during the process of adipogenesis. Pigment epithelium-derived factor, a soluble molecule with potent antiangiogenic properties, was found to be highly secreted by preadipocytes but not adipocytes. Conversely, we found hippocampal cholinergic neurostimulating peptide, neutrophil gelatinase-associated lipocalin, and haptoglobin to be expressed highly by mature adipocytes. We also used liquid chromatography-based separation followed by automated tandem mass spectrometry to identify proteins secreted by mature adipocytes. Several additional secreted proteins including resistin, secreted acidic cysteine-rich glycoprotein/osteonectin, stromal cell-derived factor-1, cystatin C, gelsolin, and matrix metalloprotease-2 were identified by this method. To our knowledge, this is the first study to identify several novel secreted proteins by adipocytes by a proteomic approach using mass spectrometry.",
keywords = "3T3 Cells, Acute-Phase Proteins, Adipocytes, Animals, Cell Differentiation, Eye Proteins, Gene Expression, Haptoglobins, Lipocalins, Mice, Nerve Growth Factors, Neuropeptides, Oncogene Proteins, Proteins, Proteome, Serpins, Spectrometry, Mass, Electrospray Ionization",
author = "Irina Kratchmarova and Kalume, {Dario E} and Blagoy Blagoev and Scherer, {Philipp E} and Podtelejnikov, {Alexandre V} and Henrik Molina and Bickel, {Perry E} and Andersen, {Jens S} and Fernandez, {Minerva M} and Jacob Bunkenborg and Peter Roepstorff and Karsten Kristiansen and Lodish, {Harvey F} and Matthias Mann and Akhilesh Pandey",
year = "2002",
month = mar,
day = "1",
language = "English",
volume = "1",
pages = "213--22",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "3",
}