A microoptode array for fine-scale measurement of oxygen distribution

Gerhard Holst*, Ronnie N. Glud, Michael Kühl, Ingo Klimant

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Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A new microoptode array is presented that provides simultaneous measurement with eight oxygen microoptodes using a simple optical setup and a phase-angle detection principle. The measuring system consists of: (1) an optical unit with eight oxygen microoptodes, a special fiber-coupler array, optical filters, lenses, light sources (light-emitting diodes) and light detectors (photodiodes, photomultiplier tube); (2) a signal-processing unit with analog signal processing (phase-angle detection, filtering) and digital signal processing (control, data storage and display). The oxygen concentration is measured with tapered silica-glass fibers (tip diameter 20-30 μm) by the dynamic quenching of a luminophore. A phase-modulation technique is used to determine the phase-angle shift that is caused by the fluorescence lifetime when the indicator is excited sinusoidally. In a time multiplex mode each sensor signal is sampled. This multisensor array system is designed for the investigation of the oxygen distribution in biofilms and aquatic sediments. The new measuring system and first applications in artificial and natural systems are presented.

OriginalsprogEngelsk
TidsskriftSensors and Actuators, B: Chemical
Vol/bind38
Udgave nummer1-3
Sider (fra-til)122-129
Antal sider8
ISSN0925-4005
DOI
StatusUdgivet - 1. jan. 1997
Udgivet eksterntJa

Fingeraftryk

Oxygen
signal processing
phase shift
oxygen
Signal processing
biofilms
Optical filters
Photomultipliers
Phase modulation
optical filters
silica glass
Biofilms
photomultiplier tubes
glass fibers
Fused silica
data storage
Photodetectors
Photodiodes
Digital signal processing
phase modulation

Citer dette

Holst, Gerhard ; Glud, Ronnie N. ; Kühl, Michael ; Klimant, Ingo. / A microoptode array for fine-scale measurement of oxygen distribution. I: Sensors and Actuators, B: Chemical. 1997 ; Bind 38, Nr. 1-3. s. 122-129.
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A microoptode array for fine-scale measurement of oxygen distribution. / Holst, Gerhard; Glud, Ronnie N.; Kühl, Michael; Klimant, Ingo.

I: Sensors and Actuators, B: Chemical, Bind 38, Nr. 1-3, 01.01.1997, s. 122-129.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

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AU - Holst, Gerhard

AU - Glud, Ronnie N.

AU - Kühl, Michael

AU - Klimant, Ingo

PY - 1997/1/1

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N2 - A new microoptode array is presented that provides simultaneous measurement with eight oxygen microoptodes using a simple optical setup and a phase-angle detection principle. The measuring system consists of: (1) an optical unit with eight oxygen microoptodes, a special fiber-coupler array, optical filters, lenses, light sources (light-emitting diodes) and light detectors (photodiodes, photomultiplier tube); (2) a signal-processing unit with analog signal processing (phase-angle detection, filtering) and digital signal processing (control, data storage and display). The oxygen concentration is measured with tapered silica-glass fibers (tip diameter 20-30 μm) by the dynamic quenching of a luminophore. A phase-modulation technique is used to determine the phase-angle shift that is caused by the fluorescence lifetime when the indicator is excited sinusoidally. In a time multiplex mode each sensor signal is sampled. This multisensor array system is designed for the investigation of the oxygen distribution in biofilms and aquatic sediments. The new measuring system and first applications in artificial and natural systems are presented.

AB - A new microoptode array is presented that provides simultaneous measurement with eight oxygen microoptodes using a simple optical setup and a phase-angle detection principle. The measuring system consists of: (1) an optical unit with eight oxygen microoptodes, a special fiber-coupler array, optical filters, lenses, light sources (light-emitting diodes) and light detectors (photodiodes, photomultiplier tube); (2) a signal-processing unit with analog signal processing (phase-angle detection, filtering) and digital signal processing (control, data storage and display). The oxygen concentration is measured with tapered silica-glass fibers (tip diameter 20-30 μm) by the dynamic quenching of a luminophore. A phase-modulation technique is used to determine the phase-angle shift that is caused by the fluorescence lifetime when the indicator is excited sinusoidally. In a time multiplex mode each sensor signal is sampled. This multisensor array system is designed for the investigation of the oxygen distribution in biofilms and aquatic sediments. The new measuring system and first applications in artificial and natural systems are presented.

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KW - Fluorescence quenching

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KW - Oxygen microsensors

KW - Oxygen optodes

KW - Phase modulation

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