Resumé
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of the American Chemical Society |
| Vol/bind | 135 |
| Udgave nummer | 7 |
| Sider (fra-til) | 2423-2426 |
| Antal sider | 4 |
| ISSN | 0002-7863 |
| DOI | |
| Status | Udgivet - 2013 |
Fingeraftryk
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A locked nucleic Acid-based nanocrawler : designed and reversible movement detected by multicolor fluorescence. / Astakhova, I Kira; Pasternak, Karol; Campbell, Meghan A; Gupta, Pankaj; Wengel, Jesper.
I: Journal of the American Chemical Society, Bind 135, Nr. 7, 2013, s. 2423-2426.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - A locked nucleic Acid-based nanocrawler
T2 - designed and reversible movement detected by multicolor fluorescence
AU - Astakhova, I Kira
AU - Pasternak, Karol
AU - Campbell, Meghan A
AU - Gupta, Pankaj
AU - Wengel, Jesper
PY - 2013
Y1 - 2013
N2 - Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
AB - Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
U2 - 10.1021/ja311250w
DO - 10.1021/ja311250w
M3 - Journal article
C2 - 23379691
VL - 135
SP - 2423
EP - 2426
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 7
ER -