Abstrakt
Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
Originalsprog | Engelsk |
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Tidsskrift | Journal of the American Chemical Society |
Vol/bind | 135 |
Udgave nummer | 7 |
Sider (fra-til) | 2423-2426 |
Antal sider | 4 |
ISSN | 0002-7863 |
DOI | |
Status | Udgivet - 2013 |