A locked nucleic Acid-based nanocrawler: designed and reversible movement detected by multicolor fluorescence

I Kira Astakhova, Karol Pasternak, Meghan A Campbell, Pankaj Gupta, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
OriginalsprogEngelsk
TidsskriftJournal of the American Chemical Society
Vol/bind135
Udgave nummer7
Sider (fra-til)2423-2426
Antal sider4
ISSN0002-7863
DOI
StatusUdgivet - 2013

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