A locked nucleic Acid-based nanocrawler: designed and reversible movement detected by multicolor fluorescence

I Kira Astakhova, Karol Pasternak, Meghan A Campbell, Pankaj Gupta, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
OriginalsprogEngelsk
TidsskriftJournal of the American Chemical Society
Vol/bind135
Udgave nummer7
Sider (fra-til)2423-2426
Antal sider4
ISSN0002-7863
DOI
StatusUdgivet - 2013

Fingeraftryk

Nucleic acids
Fluorescence
Pyrene
Hydrocarbons
Monomers
Perylene
Energy transfer
Communication systems
DNA
Coloring Agents
Dyes
Color
locked nucleic acid
pyrene

Citer dette

Astakhova, I Kira ; Pasternak, Karol ; Campbell, Meghan A ; Gupta, Pankaj ; Wengel, Jesper. / A locked nucleic Acid-based nanocrawler : designed and reversible movement detected by multicolor fluorescence. I: Journal of the American Chemical Society. 2013 ; Bind 135, Nr. 7. s. 2423-2426.
@article{bff47dcd25ea485dbf738d221e1ae776,
title = "A locked nucleic Acid-based nanocrawler: designed and reversible movement detected by multicolor fluorescence",
abstract = "Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand F{\"o}rster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.",
author = "Astakhova, {I Kira} and Karol Pasternak and Campbell, {Meghan A} and Pankaj Gupta and Jesper Wengel",
year = "2013",
doi = "10.1021/ja311250w",
language = "English",
volume = "135",
pages = "2423--2426",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "ACS Publications",
number = "7",

}

A locked nucleic Acid-based nanocrawler : designed and reversible movement detected by multicolor fluorescence. / Astakhova, I Kira; Pasternak, Karol; Campbell, Meghan A; Gupta, Pankaj; Wengel, Jesper.

I: Journal of the American Chemical Society, Bind 135, Nr. 7, 2013, s. 2423-2426.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A locked nucleic Acid-based nanocrawler

T2 - designed and reversible movement detected by multicolor fluorescence

AU - Astakhova, I Kira

AU - Pasternak, Karol

AU - Campbell, Meghan A

AU - Gupta, Pankaj

AU - Wengel, Jesper

PY - 2013

Y1 - 2013

N2 - Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.

AB - Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2'-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene-perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.

U2 - 10.1021/ja311250w

DO - 10.1021/ja311250w

M3 - Journal article

C2 - 23379691

VL - 135

SP - 2423

EP - 2426

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 7

ER -