A human gut microbial gene catalogue established by metagenomic sequencing

Junjie Qin; Ruiqiang Li; Jeroen Raes; Manimozhiyan Arumugam; Kristoffer Solvsten Burgdorf; Chaysavanh Manichanh; Trine Nielsen; Nicolas Pons; Florence Levenez; Takuji Yamada; Daniel R Mende; Junhua Li; Junming Xu; Shaochuan Li; Dongfang Li; Jianjun Cao; Bo Wang; Huiqing Liang; Huisong Zheng; Yinlong Xie; Julien Tap; Patricia Lepage; Marcelo Bertalan; Jean-Michel Batto; Torben Hansen; Denis Le Paslier; Allan Linneberg; H Bjørn Nielsen; Eric Pelletier; Pierre Renault; Thomas Sicheritz-Ponten; Keith Turner; Hongmei Zhu; Chang Yu; Shengting Li; Min Jian; Yan Zhou; Yingrui Li; Xiuqing Zhang; Songgang Li; Nan Qin; Huanming Yang; Jian Wang; Søren Brunak; Joel Doré; Francisco Guarner; Karsten Kristiansen; Oluf Pedersen; Julian Parkhill; Jean Weissenbach; Peer Bork; S Dusko Ehrlich; Jun Wang

doi:10.1038/nature08821

A human gut microbial gene catalogue established by metagenomic sequencing

Junjie Qin
, Ruiqiang Li
, Jeroen Raes
, Manimozhiyan Arumugam
, Kristoffer Solvsten Burgdorf
, Chaysavanh Manichanh
, Trine Nielsen
, Nicolas Pons
, Florence Levenez
, Takuji Yamada
, Daniel R Mende
, Junhua Li
, Junming Xu
, Shaochuan Li
, Dongfang Li
, Jianjun Cao
, Bo Wang
, Huiqing Liang
, Huisong Zheng
, Yinlong Xie

Julien Tap, Patricia Lepage, Marcelo Bertalan, Jean-Michel Batto, Torben Hansen, Denis Le Paslier, Allan Linneberg, H Bjørn Nielsen, Eric Pelletier, Pierre Renault, Thomas Sicheritz-Ponten, Keith Turner, Hongmei Zhu, Chang Yu, Shengting Li, Min Jian, Yan Zhou, Yingrui Li, Xiuqing Zhang, Songgang Li, Nan Qin, Huanming Yang, Jian Wang, Søren Brunak, Joel Doré, Francisco Guarner, Karsten Kristiansen, Oluf Pedersen, Julian Parkhill, Jean Weissenbach, Peer Bork, S Dusko Ehrlich, Jun Wang

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Udgivelsesdato: 2010-Mar-4

Originalsprog	Engelsk
Tidsskrift	Nature
Vol/bind	464
Udgave nummer	7285
Sider (fra-til)	59-65
Antal sider	6
ISSN	0028-0836
DOI	https://doi.org/10.1038/nature08821
Status	Udgivet - 4. mar. 2010

Dokumenter og links

10.1038/nature08821

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Tjek adgangen til materialet i Syddansk Universitetsbiblioteks søgemaskine

Citationsformater

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title = "A human gut microbial gene catalogue established by metagenomic sequencing",

abstract = "To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99\% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.",

keywords = "Adult, Bacteria, Cohort Studies, Contig Mapping, Denmark, Feces, Gastrointestinal Tract, Genes, Bacterial, Genes, Essential, Genome, Bacterial, Genomics, Health, Humans, Inflammatory Bowel Diseases, Metagenome, Obesity, Open Reading Frames, Overweight, Sequence Analysis, DNA, Spain",

author = "Junjie Qin and Ruiqiang Li and Jeroen Raes and Manimozhiyan Arumugam and Burgdorf, \{Kristoffer Solvsten\} and Chaysavanh Manichanh and Trine Nielsen and Nicolas Pons and Florence Levenez and Takuji Yamada and Mende, \{Daniel R\} and Junhua Li and Junming Xu and Shaochuan Li and Dongfang Li and Jianjun Cao and Bo Wang and Huiqing Liang and Huisong Zheng and Yinlong Xie and Julien Tap and Patricia Lepage and Marcelo Bertalan and Jean-Michel Batto and Torben Hansen and \{Le Paslier\}, Denis and Allan Linneberg and Nielsen, \{H Bj{\o}rn\} and Eric Pelletier and Pierre Renault and Thomas Sicheritz-Ponten and Keith Turner and Hongmei Zhu and Chang Yu and Shengting Li and Min Jian and Yan Zhou and Yingrui Li and Xiuqing Zhang and Songgang Li and Nan Qin and Huanming Yang and Jian Wang and S{\o}ren Brunak and Joel Dor{\'e} and Francisco Guarner and Karsten Kristiansen and Oluf Pedersen and Julian Parkhill and Jean Weissenbach and Peer Bork and Ehrlich, \{S Dusko\} and Jun Wang",

year = "2010",

month = mar,

day = "4",

doi = "10.1038/nature08821",

language = "English",

volume = "464",

pages = "59--65",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "7285",

}

Qin, J, Li, R, Raes, J, Arumugam, M, Burgdorf, KS, Manichanh, C, Nielsen, T, Pons, N, Levenez, F, Yamada, T, Mende, DR, Li, J, Xu, J, Li, S, Li, D, Cao, J, Wang, B, Liang, H, Zheng, H, Xie, Y, Tap, J, Lepage, P, Bertalan, M, Batto, J-M, Hansen, T, Le Paslier, D, Linneberg, A, Nielsen, HB, Pelletier, E, Renault, P, Sicheritz-Ponten, T, Turner, K, Zhu, H, Yu, C, Li, S, Jian, M, Zhou, Y, Li, Y, Zhang, X, Li, S, Qin, N, Yang, H, Wang, J, Brunak, S, Doré, J, Guarner, F, Kristiansen, K, Pedersen, O, Parkhill, J, Weissenbach, J, Bork, P, Ehrlich, SD & Wang, J 2010, 'A human gut microbial gene catalogue established by metagenomic sequencing', Nature, bind 464, nr. 7285, s. 59-65. https://doi.org/10.1038/nature08821

TY - JOUR

T1 - A human gut microbial gene catalogue established by metagenomic sequencing

AU - Qin, Junjie

AU - Li, Ruiqiang

AU - Raes, Jeroen

AU - Arumugam, Manimozhiyan

AU - Burgdorf, Kristoffer Solvsten

AU - Manichanh, Chaysavanh

AU - Nielsen, Trine

AU - Pons, Nicolas

AU - Levenez, Florence

AU - Yamada, Takuji

AU - Mende, Daniel R

AU - Li, Junhua

AU - Xu, Junming

AU - Li, Shaochuan

AU - Li, Dongfang

AU - Cao, Jianjun

AU - Wang, Bo

AU - Liang, Huiqing

AU - Zheng, Huisong

AU - Xie, Yinlong

AU - Tap, Julien

AU - Lepage, Patricia

AU - Bertalan, Marcelo

AU - Batto, Jean-Michel

AU - Hansen, Torben

AU - Le Paslier, Denis

AU - Linneberg, Allan

AU - Nielsen, H Bjørn

AU - Pelletier, Eric

AU - Renault, Pierre

AU - Sicheritz-Ponten, Thomas

AU - Turner, Keith

AU - Zhu, Hongmei

AU - Yu, Chang

AU - Li, Shengting

AU - Jian, Min

AU - Zhou, Yan

AU - Li, Yingrui

AU - Zhang, Xiuqing

AU - Li, Songgang

AU - Qin, Nan

AU - Yang, Huanming

AU - Wang, Jian

AU - Brunak, Søren

AU - Doré, Joel

AU - Guarner, Francisco

AU - Kristiansen, Karsten

AU - Pedersen, Oluf

AU - Parkhill, Julian

AU - Weissenbach, Jean

AU - Bork, Peer

AU - Ehrlich, S Dusko

AU - Wang, Jun

PY - 2010/3/4

Y1 - 2010/3/4

N2 - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

AB - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

KW - Adult

KW - Bacteria

KW - Cohort Studies

KW - Contig Mapping

KW - Denmark

KW - Feces

KW - Gastrointestinal Tract

KW - Genes, Bacterial

KW - Genes, Essential

KW - Genome, Bacterial

KW - Genomics

KW - Health

KW - Humans

KW - Inflammatory Bowel Diseases

KW - Metagenome

KW - Obesity

KW - Open Reading Frames

KW - Overweight

KW - Sequence Analysis, DNA

KW - Spain

U2 - 10.1038/nature08821

DO - 10.1038/nature08821

M3 - Journal article

C2 - 20203603

SN - 0028-0836

VL - 464

SP - 59

EP - 65

JO - Nature

JF - Nature

IS - 7285

ER -

A human gut microbial gene catalogue established by metagenomic sequencing

Abstract

Dokumenter og links

SDU link

Fingeraftryk

Citationsformater