A global benchmark study using affinity-based biosensors

Rebecca L Rich, Giuseppe A Papalia, Peter J Flynn, Jamie Furneisen, John Quinn, Joshua S Klein, Phini S Katsamba, M Brent Waddell, Michael Scott, Joshua Thompson, Judie Berlier, Schuyler Corry, Mireille Baltzinger, Gabrielle Zeder-Lutz, Andreas Schoenemann, Anca Clabbers, Sebastien Wieckowski, Mary M Murphy, Phillip Page, Thomas E Ryan & 131 andre Jay Duffner, Tanmoy Ganguly, John Corbin, Satyen Gautam, Gregor Anderluh, Andrej Bavdek, Dana Reichmann, Satya P Yadav, Eric Hommema, Ewa Pol, Andrew Drake, Scott Klakamp, Trevor Chapman, Dawn Kernaghan, Ken Miller, Jason Schuman, Kevin Lindquist, Kara Herlihy, Michael B Murphy, Richard Bohnsack, Bruce Andrien, Pietro Brandani, Danny Terwey, Rohn Millican, Ryan J Darling, Liann Wang, Quincy Carter, Joe Dotzlaf, Jacinto Lopez-Sagaseta, Islay Campbell, Paola Torreri, Sylviane Hoos, Patrick England, Yang Liu, Yasmina Abdiche, Daniel Malashock, Alanna Pinkerton, Melanie Wong, Eileen Lafer, Cynthia Hinck, Kevin Thompson, Carmelo Di Primo, Alison Joyce, Jonathan Brooks, Federico Torta, Anne Birgitte Bagge Hagel, Janus Krarup, Jesper Pass, Monica Ferreira, Sergei Shikov, Malgorzata Mikolajczyk, Yuki Abe, Gaetano Barbato, Anthony M Giannetti, Ganeshram Krishnamoorthy, Bianca Beusink, Daulet Satpaev, Tiffany Tsang, Eric Fang, James Partridge, Stephen Brohawn, James Horn, Otto Pritsch, Gonzalo Obal, Sanjay Nilapwar, Ben Busby, Gerardo Gutierrez-Sanchez, Ruchira Das Gupta, Sylvie Canepa, Krista Witte, Zaneta Nikolovska-Coleska, Yun Hee Cho, Roberta D'Agata, Kristian Schlick, Rosy Calvert, Eva M Munoz, Maria Jose Hernaiz, Tsafir Bravman, Monica Dines, Min-Hsiang Yang, Agnes Puskas, Erica Boni, Jiejin Li, Martin Wear, Asya Grinberg, Jason Baardsnes, Olan Dolezal, Melicia Gainey, Henrik Anderson, Jinlin Peng, Mark Lewis, Peter Spies, Quyhn Trinh, Sergei Bibikov, Jill Raymond, Mohammed Yousef, Vidya Chandrasekaran, Yuguo Feng, Anne Emerick, Suparna Mundodo, Rejane Guimaraes, Katy McGirr, Yue-Ji Li, Heather Hughes, Hubert Mantz, Rostislav Skrabana, Mark Witmer, Joshua Ballard, Loic Martin, Petr Skladal, George Korza, Ite Laird-Offringa, Charlene S Lee, Abdelkrim Khadir, Frank Podlaski, Phillippe Neuner, Julie Rothacker, Ashique Rafique, Nico Dankbar, Peter Kainz, Erk Gedig, Momchilo Vuyisich, Christina Boozer, Nguyen Ly, Mark Toews, Aykut Uren, Oleksandr Kalyuzhniy, Kenneth Lewis, Eugene Chomey, Brian J Pak, David G Myszka

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2009-Mar-15
OriginalsprogEngelsk
TidsskriftAnalytical Biochemistry
Vol/bind386
Udgave nummer2
Sider (fra-til)194-216
Antal sider22
ISSN0003-2697
DOI
StatusUdgivet - 15. mar. 2009

Fingeraftryk

Benchmarking
Biosensing Techniques
Biosensors
Rate constants
Assays
Proteins
Immobilized Proteins
Glutathione Transferase
Kinetic parameters
Immobilization
Ligands
Antigens
Kinetics

Citer dette

Rich, R. L., Papalia, G. A., Flynn, P. J., Furneisen, J., Quinn, J., Klein, J. S., ... Myszka, D. G. (2009). A global benchmark study using affinity-based biosensors. Analytical Biochemistry, 386(2), 194-216. https://doi.org/10.1016/j.ab.2008.11.021
Rich, Rebecca L ; Papalia, Giuseppe A ; Flynn, Peter J ; Furneisen, Jamie ; Quinn, John ; Klein, Joshua S ; Katsamba, Phini S ; Waddell, M Brent ; Scott, Michael ; Thompson, Joshua ; Berlier, Judie ; Corry, Schuyler ; Baltzinger, Mireille ; Zeder-Lutz, Gabrielle ; Schoenemann, Andreas ; Clabbers, Anca ; Wieckowski, Sebastien ; Murphy, Mary M ; Page, Phillip ; Ryan, Thomas E ; Duffner, Jay ; Ganguly, Tanmoy ; Corbin, John ; Gautam, Satyen ; Anderluh, Gregor ; Bavdek, Andrej ; Reichmann, Dana ; Yadav, Satya P ; Hommema, Eric ; Pol, Ewa ; Drake, Andrew ; Klakamp, Scott ; Chapman, Trevor ; Kernaghan, Dawn ; Miller, Ken ; Schuman, Jason ; Lindquist, Kevin ; Herlihy, Kara ; Murphy, Michael B ; Bohnsack, Richard ; Andrien, Bruce ; Brandani, Pietro ; Terwey, Danny ; Millican, Rohn ; Darling, Ryan J ; Wang, Liann ; Carter, Quincy ; Dotzlaf, Joe ; Lopez-Sagaseta, Jacinto ; Campbell, Islay ; Torreri, Paola ; Hoos, Sylviane ; England, Patrick ; Liu, Yang ; Abdiche, Yasmina ; Malashock, Daniel ; Pinkerton, Alanna ; Wong, Melanie ; Lafer, Eileen ; Hinck, Cynthia ; Thompson, Kevin ; Primo, Carmelo Di ; Joyce, Alison ; Brooks, Jonathan ; Torta, Federico ; Bagge Hagel, Anne Birgitte ; Krarup, Janus ; Pass, Jesper ; Ferreira, Monica ; Shikov, Sergei ; Mikolajczyk, Malgorzata ; Abe, Yuki ; Barbato, Gaetano ; Giannetti, Anthony M ; Krishnamoorthy, Ganeshram ; Beusink, Bianca ; Satpaev, Daulet ; Tsang, Tiffany ; Fang, Eric ; Partridge, James ; Brohawn, Stephen ; Horn, James ; Pritsch, Otto ; Obal, Gonzalo ; Nilapwar, Sanjay ; Busby, Ben ; Gutierrez-Sanchez, Gerardo ; Gupta, Ruchira Das ; Canepa, Sylvie ; Witte, Krista ; Nikolovska-Coleska, Zaneta ; Cho, Yun Hee ; D'Agata, Roberta ; Schlick, Kristian ; Calvert, Rosy ; Munoz, Eva M ; Hernaiz, Maria Jose ; Bravman, Tsafir ; Dines, Monica ; Yang, Min-Hsiang ; Puskas, Agnes ; Boni, Erica ; Li, Jiejin ; Wear, Martin ; Grinberg, Asya ; Baardsnes, Jason ; Dolezal, Olan ; Gainey, Melicia ; Anderson, Henrik ; Peng, Jinlin ; Lewis, Mark ; Spies, Peter ; Trinh, Quyhn ; Bibikov, Sergei ; Raymond, Jill ; Yousef, Mohammed ; Chandrasekaran, Vidya ; Feng, Yuguo ; Emerick, Anne ; Mundodo, Suparna ; Guimaraes, Rejane ; McGirr, Katy ; Li, Yue-Ji ; Hughes, Heather ; Mantz, Hubert ; Skrabana, Rostislav ; Witmer, Mark ; Ballard, Joshua ; Martin, Loic ; Skladal, Petr ; Korza, George ; Laird-Offringa, Ite ; Lee, Charlene S ; Khadir, Abdelkrim ; Podlaski, Frank ; Neuner, Phillippe ; Rothacker, Julie ; Rafique, Ashique ; Dankbar, Nico ; Kainz, Peter ; Gedig, Erk ; Vuyisich, Momchilo ; Boozer, Christina ; Ly, Nguyen ; Toews, Mark ; Uren, Aykut ; Kalyuzhniy, Oleksandr ; Lewis, Kenneth ; Chomey, Eugene ; Pak, Brian J ; Myszka, David G. / A global benchmark study using affinity-based biosensors. I: Analytical Biochemistry. 2009 ; Bind 386, Nr. 2. s. 194-216.
@article{cbacd8b00c2511dfaefb000ea68e967b,
title = "A global benchmark study using affinity-based biosensors",
abstract = "To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.",
keywords = "Antibodies, Catalytic, Benchmarking, Binding Sites, Biosensing Techniques, Glutathione Transferase, Kinetics, Ligands, Proteins",
author = "Rich, {Rebecca L} and Papalia, {Giuseppe A} and Flynn, {Peter J} and Jamie Furneisen and John Quinn and Klein, {Joshua S} and Katsamba, {Phini S} and Waddell, {M Brent} and Michael Scott and Joshua Thompson and Judie Berlier and Schuyler Corry and Mireille Baltzinger and Gabrielle Zeder-Lutz and Andreas Schoenemann and Anca Clabbers and Sebastien Wieckowski and Murphy, {Mary M} and Phillip Page and Ryan, {Thomas E} and Jay Duffner and Tanmoy Ganguly and John Corbin and Satyen Gautam and Gregor Anderluh and Andrej Bavdek and Dana Reichmann and Yadav, {Satya P} and Eric Hommema and Ewa Pol and Andrew Drake and Scott Klakamp and Trevor Chapman and Dawn Kernaghan and Ken Miller and Jason Schuman and Kevin Lindquist and Kara Herlihy and Murphy, {Michael B} and Richard Bohnsack and Bruce Andrien and Pietro Brandani and Danny Terwey and Rohn Millican and Darling, {Ryan J} and Liann Wang and Quincy Carter and Joe Dotzlaf and Jacinto Lopez-Sagaseta and Islay Campbell and Paola Torreri and Sylviane Hoos and Patrick England and Yang Liu and Yasmina Abdiche and Daniel Malashock and Alanna Pinkerton and Melanie Wong and Eileen Lafer and Cynthia Hinck and Kevin Thompson and Primo, {Carmelo Di} and Alison Joyce and Jonathan Brooks and Federico Torta and {Bagge Hagel}, {Anne Birgitte} and Janus Krarup and Jesper Pass and Monica Ferreira and Sergei Shikov and Malgorzata Mikolajczyk and Yuki Abe and Gaetano Barbato and Giannetti, {Anthony M} and Ganeshram Krishnamoorthy and Bianca Beusink and Daulet Satpaev and Tiffany Tsang and Eric Fang and James Partridge and Stephen Brohawn and James Horn and Otto Pritsch and Gonzalo Obal and Sanjay Nilapwar and Ben Busby and Gerardo Gutierrez-Sanchez and Gupta, {Ruchira Das} and Sylvie Canepa and Krista Witte and Zaneta Nikolovska-Coleska and Cho, {Yun Hee} and Roberta D'Agata and Kristian Schlick and Rosy Calvert and Munoz, {Eva M} and Hernaiz, {Maria Jose} and Tsafir Bravman and Monica Dines and Min-Hsiang Yang and Agnes Puskas and Erica Boni and Jiejin Li and Martin Wear and Asya Grinberg and Jason Baardsnes and Olan Dolezal and Melicia Gainey and Henrik Anderson and Jinlin Peng and Mark Lewis and Peter Spies and Quyhn Trinh and Sergei Bibikov and Jill Raymond and Mohammed Yousef and Vidya Chandrasekaran and Yuguo Feng and Anne Emerick and Suparna Mundodo and Rejane Guimaraes and Katy McGirr and Yue-Ji Li and Heather Hughes and Hubert Mantz and Rostislav Skrabana and Mark Witmer and Joshua Ballard and Loic Martin and Petr Skladal and George Korza and Ite Laird-Offringa and Lee, {Charlene S} and Abdelkrim Khadir and Frank Podlaski and Phillippe Neuner and Julie Rothacker and Ashique Rafique and Nico Dankbar and Peter Kainz and Erk Gedig and Momchilo Vuyisich and Christina Boozer and Nguyen Ly and Mark Toews and Aykut Uren and Oleksandr Kalyuzhniy and Kenneth Lewis and Eugene Chomey and Pak, {Brian J} and Myszka, {David G}",
year = "2009",
month = "3",
day = "15",
doi = "10.1016/j.ab.2008.11.021",
language = "English",
volume = "386",
pages = "194--216",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Elsevier",
number = "2",

}

Rich, RL, Papalia, GA, Flynn, PJ, Furneisen, J, Quinn, J, Klein, JS, Katsamba, PS, Waddell, MB, Scott, M, Thompson, J, Berlier, J, Corry, S, Baltzinger, M, Zeder-Lutz, G, Schoenemann, A, Clabbers, A, Wieckowski, S, Murphy, MM, Page, P, Ryan, TE, Duffner, J, Ganguly, T, Corbin, J, Gautam, S, Anderluh, G, Bavdek, A, Reichmann, D, Yadav, SP, Hommema, E, Pol, E, Drake, A, Klakamp, S, Chapman, T, Kernaghan, D, Miller, K, Schuman, J, Lindquist, K, Herlihy, K, Murphy, MB, Bohnsack, R, Andrien, B, Brandani, P, Terwey, D, Millican, R, Darling, RJ, Wang, L, Carter, Q, Dotzlaf, J, Lopez-Sagaseta, J, Campbell, I, Torreri, P, Hoos, S, England, P, Liu, Y, Abdiche, Y, Malashock, D, Pinkerton, A, Wong, M, Lafer, E, Hinck, C, Thompson, K, Primo, CD, Joyce, A, Brooks, J, Torta, F, Bagge Hagel, AB, Krarup, J, Pass, J, Ferreira, M, Shikov, S, Mikolajczyk, M, Abe, Y, Barbato, G, Giannetti, AM, Krishnamoorthy, G, Beusink, B, Satpaev, D, Tsang, T, Fang, E, Partridge, J, Brohawn, S, Horn, J, Pritsch, O, Obal, G, Nilapwar, S, Busby, B, Gutierrez-Sanchez, G, Gupta, RD, Canepa, S, Witte, K, Nikolovska-Coleska, Z, Cho, YH, D'Agata, R, Schlick, K, Calvert, R, Munoz, EM, Hernaiz, MJ, Bravman, T, Dines, M, Yang, M-H, Puskas, A, Boni, E, Li, J, Wear, M, Grinberg, A, Baardsnes, J, Dolezal, O, Gainey, M, Anderson, H, Peng, J, Lewis, M, Spies, P, Trinh, Q, Bibikov, S, Raymond, J, Yousef, M, Chandrasekaran, V, Feng, Y, Emerick, A, Mundodo, S, Guimaraes, R, McGirr, K, Li, Y-J, Hughes, H, Mantz, H, Skrabana, R, Witmer, M, Ballard, J, Martin, L, Skladal, P, Korza, G, Laird-Offringa, I, Lee, CS, Khadir, A, Podlaski, F, Neuner, P, Rothacker, J, Rafique, A, Dankbar, N, Kainz, P, Gedig, E, Vuyisich, M, Boozer, C, Ly, N, Toews, M, Uren, A, Kalyuzhniy, O, Lewis, K, Chomey, E, Pak, BJ & Myszka, DG 2009, 'A global benchmark study using affinity-based biosensors', Analytical Biochemistry, bind 386, nr. 2, s. 194-216. https://doi.org/10.1016/j.ab.2008.11.021

A global benchmark study using affinity-based biosensors. / Rich, Rebecca L; Papalia, Giuseppe A; Flynn, Peter J; Furneisen, Jamie; Quinn, John; Klein, Joshua S; Katsamba, Phini S; Waddell, M Brent; Scott, Michael; Thompson, Joshua; Berlier, Judie; Corry, Schuyler; Baltzinger, Mireille; Zeder-Lutz, Gabrielle; Schoenemann, Andreas; Clabbers, Anca; Wieckowski, Sebastien; Murphy, Mary M; Page, Phillip; Ryan, Thomas E; Duffner, Jay; Ganguly, Tanmoy; Corbin, John; Gautam, Satyen; Anderluh, Gregor; Bavdek, Andrej; Reichmann, Dana; Yadav, Satya P; Hommema, Eric; Pol, Ewa; Drake, Andrew; Klakamp, Scott; Chapman, Trevor; Kernaghan, Dawn; Miller, Ken; Schuman, Jason; Lindquist, Kevin; Herlihy, Kara; Murphy, Michael B; Bohnsack, Richard; Andrien, Bruce; Brandani, Pietro; Terwey, Danny; Millican, Rohn; Darling, Ryan J; Wang, Liann; Carter, Quincy; Dotzlaf, Joe; Lopez-Sagaseta, Jacinto; Campbell, Islay; Torreri, Paola; Hoos, Sylviane; England, Patrick; Liu, Yang; Abdiche, Yasmina; Malashock, Daniel; Pinkerton, Alanna; Wong, Melanie; Lafer, Eileen; Hinck, Cynthia; Thompson, Kevin; Primo, Carmelo Di; Joyce, Alison; Brooks, Jonathan; Torta, Federico; Bagge Hagel, Anne Birgitte; Krarup, Janus; Pass, Jesper; Ferreira, Monica; Shikov, Sergei; Mikolajczyk, Malgorzata; Abe, Yuki; Barbato, Gaetano; Giannetti, Anthony M; Krishnamoorthy, Ganeshram; Beusink, Bianca; Satpaev, Daulet; Tsang, Tiffany; Fang, Eric; Partridge, James; Brohawn, Stephen; Horn, James; Pritsch, Otto; Obal, Gonzalo; Nilapwar, Sanjay; Busby, Ben; Gutierrez-Sanchez, Gerardo; Gupta, Ruchira Das; Canepa, Sylvie; Witte, Krista; Nikolovska-Coleska, Zaneta; Cho, Yun Hee; D'Agata, Roberta; Schlick, Kristian; Calvert, Rosy; Munoz, Eva M; Hernaiz, Maria Jose; Bravman, Tsafir; Dines, Monica; Yang, Min-Hsiang; Puskas, Agnes; Boni, Erica; Li, Jiejin; Wear, Martin; Grinberg, Asya; Baardsnes, Jason; Dolezal, Olan; Gainey, Melicia; Anderson, Henrik; Peng, Jinlin; Lewis, Mark; Spies, Peter; Trinh, Quyhn; Bibikov, Sergei; Raymond, Jill; Yousef, Mohammed; Chandrasekaran, Vidya; Feng, Yuguo; Emerick, Anne; Mundodo, Suparna; Guimaraes, Rejane; McGirr, Katy; Li, Yue-Ji; Hughes, Heather; Mantz, Hubert; Skrabana, Rostislav; Witmer, Mark; Ballard, Joshua; Martin, Loic; Skladal, Petr; Korza, George; Laird-Offringa, Ite; Lee, Charlene S; Khadir, Abdelkrim; Podlaski, Frank; Neuner, Phillippe; Rothacker, Julie; Rafique, Ashique; Dankbar, Nico; Kainz, Peter; Gedig, Erk; Vuyisich, Momchilo; Boozer, Christina; Ly, Nguyen; Toews, Mark; Uren, Aykut; Kalyuzhniy, Oleksandr; Lewis, Kenneth; Chomey, Eugene; Pak, Brian J; Myszka, David G.

I: Analytical Biochemistry, Bind 386, Nr. 2, 15.03.2009, s. 194-216.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A global benchmark study using affinity-based biosensors

AU - Rich, Rebecca L

AU - Papalia, Giuseppe A

AU - Flynn, Peter J

AU - Furneisen, Jamie

AU - Quinn, John

AU - Klein, Joshua S

AU - Katsamba, Phini S

AU - Waddell, M Brent

AU - Scott, Michael

AU - Thompson, Joshua

AU - Berlier, Judie

AU - Corry, Schuyler

AU - Baltzinger, Mireille

AU - Zeder-Lutz, Gabrielle

AU - Schoenemann, Andreas

AU - Clabbers, Anca

AU - Wieckowski, Sebastien

AU - Murphy, Mary M

AU - Page, Phillip

AU - Ryan, Thomas E

AU - Duffner, Jay

AU - Ganguly, Tanmoy

AU - Corbin, John

AU - Gautam, Satyen

AU - Anderluh, Gregor

AU - Bavdek, Andrej

AU - Reichmann, Dana

AU - Yadav, Satya P

AU - Hommema, Eric

AU - Pol, Ewa

AU - Drake, Andrew

AU - Klakamp, Scott

AU - Chapman, Trevor

AU - Kernaghan, Dawn

AU - Miller, Ken

AU - Schuman, Jason

AU - Lindquist, Kevin

AU - Herlihy, Kara

AU - Murphy, Michael B

AU - Bohnsack, Richard

AU - Andrien, Bruce

AU - Brandani, Pietro

AU - Terwey, Danny

AU - Millican, Rohn

AU - Darling, Ryan J

AU - Wang, Liann

AU - Carter, Quincy

AU - Dotzlaf, Joe

AU - Lopez-Sagaseta, Jacinto

AU - Campbell, Islay

AU - Torreri, Paola

AU - Hoos, Sylviane

AU - England, Patrick

AU - Liu, Yang

AU - Abdiche, Yasmina

AU - Malashock, Daniel

AU - Pinkerton, Alanna

AU - Wong, Melanie

AU - Lafer, Eileen

AU - Hinck, Cynthia

AU - Thompson, Kevin

AU - Primo, Carmelo Di

AU - Joyce, Alison

AU - Brooks, Jonathan

AU - Torta, Federico

AU - Bagge Hagel, Anne Birgitte

AU - Krarup, Janus

AU - Pass, Jesper

AU - Ferreira, Monica

AU - Shikov, Sergei

AU - Mikolajczyk, Malgorzata

AU - Abe, Yuki

AU - Barbato, Gaetano

AU - Giannetti, Anthony M

AU - Krishnamoorthy, Ganeshram

AU - Beusink, Bianca

AU - Satpaev, Daulet

AU - Tsang, Tiffany

AU - Fang, Eric

AU - Partridge, James

AU - Brohawn, Stephen

AU - Horn, James

AU - Pritsch, Otto

AU - Obal, Gonzalo

AU - Nilapwar, Sanjay

AU - Busby, Ben

AU - Gutierrez-Sanchez, Gerardo

AU - Gupta, Ruchira Das

AU - Canepa, Sylvie

AU - Witte, Krista

AU - Nikolovska-Coleska, Zaneta

AU - Cho, Yun Hee

AU - D'Agata, Roberta

AU - Schlick, Kristian

AU - Calvert, Rosy

AU - Munoz, Eva M

AU - Hernaiz, Maria Jose

AU - Bravman, Tsafir

AU - Dines, Monica

AU - Yang, Min-Hsiang

AU - Puskas, Agnes

AU - Boni, Erica

AU - Li, Jiejin

AU - Wear, Martin

AU - Grinberg, Asya

AU - Baardsnes, Jason

AU - Dolezal, Olan

AU - Gainey, Melicia

AU - Anderson, Henrik

AU - Peng, Jinlin

AU - Lewis, Mark

AU - Spies, Peter

AU - Trinh, Quyhn

AU - Bibikov, Sergei

AU - Raymond, Jill

AU - Yousef, Mohammed

AU - Chandrasekaran, Vidya

AU - Feng, Yuguo

AU - Emerick, Anne

AU - Mundodo, Suparna

AU - Guimaraes, Rejane

AU - McGirr, Katy

AU - Li, Yue-Ji

AU - Hughes, Heather

AU - Mantz, Hubert

AU - Skrabana, Rostislav

AU - Witmer, Mark

AU - Ballard, Joshua

AU - Martin, Loic

AU - Skladal, Petr

AU - Korza, George

AU - Laird-Offringa, Ite

AU - Lee, Charlene S

AU - Khadir, Abdelkrim

AU - Podlaski, Frank

AU - Neuner, Phillippe

AU - Rothacker, Julie

AU - Rafique, Ashique

AU - Dankbar, Nico

AU - Kainz, Peter

AU - Gedig, Erk

AU - Vuyisich, Momchilo

AU - Boozer, Christina

AU - Ly, Nguyen

AU - Toews, Mark

AU - Uren, Aykut

AU - Kalyuzhniy, Oleksandr

AU - Lewis, Kenneth

AU - Chomey, Eugene

AU - Pak, Brian J

AU - Myszka, David G

PY - 2009/3/15

Y1 - 2009/3/15

N2 - To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.

AB - To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.

KW - Antibodies, Catalytic

KW - Benchmarking

KW - Binding Sites

KW - Biosensing Techniques

KW - Glutathione Transferase

KW - Kinetics

KW - Ligands

KW - Proteins

U2 - 10.1016/j.ab.2008.11.021

DO - 10.1016/j.ab.2008.11.021

M3 - Journal article

VL - 386

SP - 194

EP - 216

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -

Rich RL, Papalia GA, Flynn PJ, Furneisen J, Quinn J, Klein JS et al. A global benchmark study using affinity-based biosensors. Analytical Biochemistry. 2009 mar 15;386(2):194-216. https://doi.org/10.1016/j.ab.2008.11.021