A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates

Chen Chen, Jiaqi Tang, Wei Dong, Changjun Wang, Youjun Feng, Jing Wang, Feng Zheng, Xiuzhen Pan, Di Liu, Ming Li, Yajun Song, Xinxing Zhu, Haibo Sun, Tao Feng, Zhaobiao Guo, Aiping Ju, Junchao Ge, Yaqing Dong, Wen Sun, Yongqiang Jiang & 8 andre Jun Wang, Jinghua Yan, Huanming Yang, Xiaoning Wang, George F Gao, Ruifu Yang, Jian Wang, Jun Yu

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2007
OriginalsprogEngelsk
TidsskriftPLoS ONE
Vol/bind2
Udgave nummer3
Sider (fra-til)e315
ISSN1932-6203
DOI
StatusUdgivet - 1. jan. 2007

Fingeraftryk

septic shock
Poisons
Septic Shock
serotypes
genomics
Genomic Islands
pathogenicity islands
Streptococcus suis
Polyamideimides
laboratory animals
infection
Pathogens
Streptococcus pyogenes
Genomics
Serogroup
meningitis
Assays
etiological agents
Streptococcus
septicemia

Citer dette

Chen, Chen ; Tang, Jiaqi ; Dong, Wei ; Wang, Changjun ; Feng, Youjun ; Wang, Jing ; Zheng, Feng ; Pan, Xiuzhen ; Liu, Di ; Li, Ming ; Song, Yajun ; Zhu, Xinxing ; Sun, Haibo ; Feng, Tao ; Guo, Zhaobiao ; Ju, Aiping ; Ge, Junchao ; Dong, Yaqing ; Sun, Wen ; Jiang, Yongqiang ; Wang, Jun ; Yan, Jinghua ; Yang, Huanming ; Wang, Xiaoning ; Gao, George F ; Yang, Ruifu ; Wang, Jian ; Yu, Jun. / A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates. I: PLoS ONE. 2007 ; Bind 2, Nr. 3. s. e315.
@article{d3fce350def211dc860c000ea68e967b,
title = "A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates",
abstract = "BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. METHODS AND FINDINGS: To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of approximately 89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. CONCLUSIONS: To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.",
author = "Chen Chen and Jiaqi Tang and Wei Dong and Changjun Wang and Youjun Feng and Jing Wang and Feng Zheng and Xiuzhen Pan and Di Liu and Ming Li and Yajun Song and Xinxing Zhu and Haibo Sun and Tao Feng and Zhaobiao Guo and Aiping Ju and Junchao Ge and Yaqing Dong and Wen Sun and Yongqiang Jiang and Jun Wang and Jinghua Yan and Huanming Yang and Xiaoning Wang and Gao, {George F} and Ruifu Yang and Jian Wang and Jun Yu",
year = "2007",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0000315",
language = "English",
volume = "2",
pages = "e315",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

Chen, C, Tang, J, Dong, W, Wang, C, Feng, Y, Wang, J, Zheng, F, Pan, X, Liu, D, Li, M, Song, Y, Zhu, X, Sun, H, Feng, T, Guo, Z, Ju, A, Ge, J, Dong, Y, Sun, W, Jiang, Y, Wang, J, Yan, J, Yang, H, Wang, X, Gao, GF, Yang, R, Wang, J & Yu, J 2007, 'A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates', PLoS ONE, bind 2, nr. 3, s. e315. https://doi.org/10.1371/journal.pone.0000315

A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates. / Chen, Chen; Tang, Jiaqi; Dong, Wei; Wang, Changjun; Feng, Youjun; Wang, Jing; Zheng, Feng; Pan, Xiuzhen; Liu, Di; Li, Ming; Song, Yajun; Zhu, Xinxing; Sun, Haibo; Feng, Tao; Guo, Zhaobiao; Ju, Aiping; Ge, Junchao; Dong, Yaqing; Sun, Wen; Jiang, Yongqiang; Wang, Jun; Yan, Jinghua; Yang, Huanming; Wang, Xiaoning; Gao, George F; Yang, Ruifu; Wang, Jian; Yu, Jun.

I: PLoS ONE, Bind 2, Nr. 3, 01.01.2007, s. e315.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates

AU - Chen, Chen

AU - Tang, Jiaqi

AU - Dong, Wei

AU - Wang, Changjun

AU - Feng, Youjun

AU - Wang, Jing

AU - Zheng, Feng

AU - Pan, Xiuzhen

AU - Liu, Di

AU - Li, Ming

AU - Song, Yajun

AU - Zhu, Xinxing

AU - Sun, Haibo

AU - Feng, Tao

AU - Guo, Zhaobiao

AU - Ju, Aiping

AU - Ge, Junchao

AU - Dong, Yaqing

AU - Sun, Wen

AU - Jiang, Yongqiang

AU - Wang, Jun

AU - Yan, Jinghua

AU - Yang, Huanming

AU - Wang, Xiaoning

AU - Gao, George F

AU - Yang, Ruifu

AU - Wang, Jian

AU - Yu, Jun

PY - 2007/1/1

Y1 - 2007/1/1

N2 - BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. METHODS AND FINDINGS: To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of approximately 89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. CONCLUSIONS: To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.

AB - BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. METHODS AND FINDINGS: To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of approximately 89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. CONCLUSIONS: To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.

U2 - 10.1371/journal.pone.0000315

DO - 10.1371/journal.pone.0000315

M3 - Journal article

VL - 2

SP - e315

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 3

ER -