A DNA-programmed liposome fusion cascade

Philipp M G Löffler, Oliver Ries, Alexander Rabe, Anders Hauge Okholm, Rasmus P Thomsen, Jørgen Kjems, Stefan Vogel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

266 Downloads (Pure)

Abstrakt

Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand spatial and temporal control over entrapped species. Here, we address this demand with a DNA-encoded design for the successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion-protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. This platform could thus enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

OriginalsprogEngelsk
TidsskriftAngewandte Chemie International Edition
Vol/bind56
Udgave nummer43
Sider (fra-til)13228–13231
ISSN1433-7851
DOI
StatusUdgivet - 16. okt. 2017

Fingeraftryk Dyk ned i forskningsemnerne om 'A DNA-programmed liposome fusion cascade'. Sammen danner de et unikt fingeraftryk.

Citationsformater