A DNA-programmed liposome fusion cascade

Philipp M G Löffler, Oliver Ries, Alexander Rabe, Anders Hauge Okholm, Rasmus P Thomsen, Jørgen Kjems, Stefan Vogel

Publikation: Bidrag til tidsskriftLetterForskningpeer review

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Resumé

Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand for spatial and temporal control over entrapped species. Here, we address this demand by a DNA-encoded design for successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding-potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. Thus, our platform will enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

OriginalsprogEngelsk
TidsskriftAngewandte Chemie International Edition
Vol/bind56
Udgave nummer43
Sider (fra-til)13228–13231
ISSN1433-7851
DOI
StatusUdgivet - 2017

Fingeraftryk

Liposomes
DNA
Fusion reactions
Unilamellar Liposomes
Oligonucleotides
Proteins
Membranes
Processing

Citer dette

Löffler, Philipp M G ; Ries, Oliver ; Rabe, Alexander ; Okholm, Anders Hauge ; Thomsen, Rasmus P ; Kjems, Jørgen ; Vogel, Stefan. / A DNA-programmed liposome fusion cascade. I: Angewandte Chemie International Edition. 2017 ; Bind 56, Nr. 43. s. 13228–13231.
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Löffler, PMG, Ries, O, Rabe, A, Okholm, AH, Thomsen, RP, Kjems, J & Vogel, S 2017, 'A DNA-programmed liposome fusion cascade', Angewandte Chemie International Edition, bind 56, nr. 43, s. 13228–13231. https://doi.org/10.1002/anie.201703243

A DNA-programmed liposome fusion cascade. / Löffler, Philipp M G; Ries, Oliver; Rabe, Alexander; Okholm, Anders Hauge; Thomsen, Rasmus P; Kjems, Jørgen; Vogel, Stefan.

I: Angewandte Chemie International Edition, Bind 56, Nr. 43, 2017, s. 13228–13231.

Publikation: Bidrag til tidsskriftLetterForskningpeer review

TY - JOUR

T1 - A DNA-programmed liposome fusion cascade

AU - Löffler, Philipp M G

AU - Ries, Oliver

AU - Rabe, Alexander

AU - Okholm, Anders Hauge

AU - Thomsen, Rasmus P

AU - Kjems, Jørgen

AU - Vogel, Stefan

N1 - © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2017

Y1 - 2017

N2 - Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand for spatial and temporal control over entrapped species. Here, we address this demand by a DNA-encoded design for successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding-potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. Thus, our platform will enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

AB - Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand for spatial and temporal control over entrapped species. Here, we address this demand by a DNA-encoded design for successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding-potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. Thus, our platform will enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

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M3 - Letter

C2 - 28598002

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JO - Angewandte Chemie International Edition

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Löffler PMG, Ries O, Rabe A, Okholm AH, Thomsen RP, Kjems J et al. A DNA-programmed liposome fusion cascade. Angewandte Chemie International Edition. 2017;56(43):13228–13231. https://doi.org/10.1002/anie.201703243