Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia

Cathy Mitchelmore, Karen M Kjaerulff, Hans C Pedersen, Jakob V Nielsen, Thomas E Rasmussen, Mads F Fisker, Bente Finsen, Karen M Pedersen, Niels A Jensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2002-Mar-1
OriginalsprogEngelsk
TidsskriftJournal of Biological Chemistry
Vol/bind277
Udgave nummer9
Sider (fra-til)7598-609
ISSN0021-9258
DOI
StatusUdgivet - 2002

Fingeraftryk

Neurons
Zinc
Protein Isoforms
Poxviridae
Proteins
Chromatin Assembly and Disassembly
Pyramidal Cells
DNA-Binding Proteins
Nuclear Proteins
Neuroglia
BTB-POZ Domain
DNA sequences
Leukemia
Bromodeoxyuridine
Labeling
Chromatin
Binding Sites
Neoplasms

Citer dette

Mitchelmore, Cathy ; Kjaerulff, Karen M ; Pedersen, Hans C ; Nielsen, Jakob V ; Rasmussen, Thomas E ; Fisker, Mads F ; Finsen, Bente ; Pedersen, Karen M ; Jensen, Niels A. / Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia. I: Journal of Biological Chemistry. 2002 ; Bind 277, Nr. 9. s. 7598-609.
@article{34c4fd40ba9911dc9626000ea68e967b,
title = "Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia",
abstract = "BTB/POZ (broad complex tramtrack bric-a-brac/poxvirus and zinc finger) zinc finger factors are a class of nuclear DNA-binding proteins involved in development, chromatin remodeling, and cancer. However, BTB/POZ domain zinc finger factors linked to development of the mammalian cerebral cortex, cerebellum, and macroglia have not been described previously. We report here the isolation and characterization of two novel nuclear BTB/POZ domain zinc finger isoforms, designated HOF(L) and HOF(S), that are specifically expressed in early hippocampal neurons, cerebellar granule cells, and gliogenic progenitors as well as in differentiated glia. During embryonic development of the murine cerebral cortex, HOF expression is restricted to the hippocampal subdivision. Expression coincides with early differentiation of presumptive CA1 and CA3 pyramidal neurons and dentate gyrus granule cells, with a sharp decline in expression at the CA1/subicular border. By using bromodeoxyuridine labeling and immunohistochemistry, we show that HOF expression coincides with immature non-dividing cells and is down-regulated in differentiated cells, suggesting a role for HOF in hippocampal neurogenesis. Consistent with the postulated role of the POZ domain as a site for protein-protein interactions, both HOF isoforms are able to dimerize. The HOF zinc fingers bind specifically to the binding site for the related promyelocytic leukemia zinc finger protein as well as to a newly identified DNA sequence.",
author = "Cathy Mitchelmore and Kjaerulff, {Karen M} and Pedersen, {Hans C} and Nielsen, {Jakob V} and Rasmussen, {Thomas E} and Fisker, {Mads F} and Bente Finsen and Pedersen, {Karen M} and Jensen, {Niels A}",
year = "2002",
doi = "10.1074/jbc.M110023200",
language = "English",
volume = "277",
pages = "7598--609",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "9",

}

Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia. / Mitchelmore, Cathy; Kjaerulff, Karen M; Pedersen, Hans C; Nielsen, Jakob V; Rasmussen, Thomas E; Fisker, Mads F; Finsen, Bente; Pedersen, Karen M; Jensen, Niels A.

I: Journal of Biological Chemistry, Bind 277, Nr. 9, 2002, s. 7598-609.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia

AU - Mitchelmore, Cathy

AU - Kjaerulff, Karen M

AU - Pedersen, Hans C

AU - Nielsen, Jakob V

AU - Rasmussen, Thomas E

AU - Fisker, Mads F

AU - Finsen, Bente

AU - Pedersen, Karen M

AU - Jensen, Niels A

PY - 2002

Y1 - 2002

N2 - BTB/POZ (broad complex tramtrack bric-a-brac/poxvirus and zinc finger) zinc finger factors are a class of nuclear DNA-binding proteins involved in development, chromatin remodeling, and cancer. However, BTB/POZ domain zinc finger factors linked to development of the mammalian cerebral cortex, cerebellum, and macroglia have not been described previously. We report here the isolation and characterization of two novel nuclear BTB/POZ domain zinc finger isoforms, designated HOF(L) and HOF(S), that are specifically expressed in early hippocampal neurons, cerebellar granule cells, and gliogenic progenitors as well as in differentiated glia. During embryonic development of the murine cerebral cortex, HOF expression is restricted to the hippocampal subdivision. Expression coincides with early differentiation of presumptive CA1 and CA3 pyramidal neurons and dentate gyrus granule cells, with a sharp decline in expression at the CA1/subicular border. By using bromodeoxyuridine labeling and immunohistochemistry, we show that HOF expression coincides with immature non-dividing cells and is down-regulated in differentiated cells, suggesting a role for HOF in hippocampal neurogenesis. Consistent with the postulated role of the POZ domain as a site for protein-protein interactions, both HOF isoforms are able to dimerize. The HOF zinc fingers bind specifically to the binding site for the related promyelocytic leukemia zinc finger protein as well as to a newly identified DNA sequence.

AB - BTB/POZ (broad complex tramtrack bric-a-brac/poxvirus and zinc finger) zinc finger factors are a class of nuclear DNA-binding proteins involved in development, chromatin remodeling, and cancer. However, BTB/POZ domain zinc finger factors linked to development of the mammalian cerebral cortex, cerebellum, and macroglia have not been described previously. We report here the isolation and characterization of two novel nuclear BTB/POZ domain zinc finger isoforms, designated HOF(L) and HOF(S), that are specifically expressed in early hippocampal neurons, cerebellar granule cells, and gliogenic progenitors as well as in differentiated glia. During embryonic development of the murine cerebral cortex, HOF expression is restricted to the hippocampal subdivision. Expression coincides with early differentiation of presumptive CA1 and CA3 pyramidal neurons and dentate gyrus granule cells, with a sharp decline in expression at the CA1/subicular border. By using bromodeoxyuridine labeling and immunohistochemistry, we show that HOF expression coincides with immature non-dividing cells and is down-regulated in differentiated cells, suggesting a role for HOF in hippocampal neurogenesis. Consistent with the postulated role of the POZ domain as a site for protein-protein interactions, both HOF isoforms are able to dimerize. The HOF zinc fingers bind specifically to the binding site for the related promyelocytic leukemia zinc finger protein as well as to a newly identified DNA sequence.

U2 - 10.1074/jbc.M110023200

DO - 10.1074/jbc.M110023200

M3 - Journal article

VL - 277

SP - 7598

EP - 7609

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 9

ER -