2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Santhosh T. Kumar; Jesper Wengel; Patrick J. Hrdlicka

doi:10.1002/cbic.200700144

2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Santhosh T. Kumar
, Jesper Wengel
, Patrick J. Hrdlicka

University of Idaho

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.

Originalsprog	Engelsk
Tidsskrift	ChemBioChem
Vol/bind	8
Udgave nummer	10
Sider (fra-til)	1122-1125
ISSN	1439-7633
DOI	https://doi.org/10.1002/cbic.200700144
Status	Udgivet - 2007

Dokumenter og links

10.1002/cbic.200700144

SDU link

Tjek adgangen til materialet i Syddansk Universitetsbiblioteks søgemaskine

Citationsformater

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abstract = "Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.",

author = "Kumar, \{Santhosh T.\} and Jesper Wengel and Hrdlicka, \{Patrick J.\}",

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AU - Wengel, Jesper

AU - Hrdlicka, Patrick J.

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AB - Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.

U2 - 10.1002/cbic.200700144

DO - 10.1002/cbic.200700144

M3 - Journal article

SN - 1439-7633

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2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Abstract

Dokumenter og links

SDU link

Fingeraftryk

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