2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Santhosh T. Kumar, Jesper Wengel, Patrick J. Hrdlicka

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.
OriginalsprogEngelsk
TidsskriftChemBioChem
Vol/bind8
Udgave nummer10
Sider (fra-til)1122-1125
ISSN1439-4227
DOI
StatusUdgivet - 2007

Fingeraftryk Dyk ned i forskningsemnerne om '2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets'. Sammen danner de et unikt fingeraftryk.

Citationsformater