2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Santhosh T. Kumar, Jesper Wengel, Patrick J. Hrdlicka

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.
OriginalsprogEngelsk
TidsskriftChemBioChem
Vol/bind8
Udgave nummer10
Sider (fra-til)1122-1125
ISSN1439-4227
DOI
StatusUdgivet - 2007

Fingeraftryk

Nucleotides
RNA
Intercalating Agents
Heptanes
DNA
Skeleton
Nucleic Acids
Monomers
pyrene
7-(2-cyanoethoxy(diisopropylamino)phosphinoxy)-1-(4,4'-dimethoxytrityloxymethyl)-5-(9'-fluorenylmethoxycarbonyl)-3-(thymin-1-yl)-2-oxa-5-azabicyclo(2.2.1)heptane

Citer dette

@article{5c999650def711dc860c000ea68e967b,
title = "2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets",
abstract = "Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.",
author = "Kumar, {Santhosh T.} and Jesper Wengel and Hrdlicka, {Patrick J.}",
year = "2007",
doi = "10.1002/cbic.200700144",
language = "English",
volume = "8",
pages = "1122--1125",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "10",

}

2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets. / Kumar, Santhosh T.; Wengel, Jesper; Hrdlicka, Patrick J.

I: ChemBioChem, Bind 8, Nr. 10, 2007, s. 1122-1125.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - 2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

AU - Kumar, Santhosh T.

AU - Wengel, Jesper

AU - Hrdlicka, Patrick J.

PY - 2007

Y1 - 2007

N2 - Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.

AB - Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.

U2 - 10.1002/cbic.200700144

DO - 10.1002/cbic.200700144

M3 - Journal article

VL - 8

SP - 1122

EP - 1125

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 10

ER -