Submicron and micron particles forming in aqueous medium upon dispersion of amorphous solid dispersions of the poorly soluble drug ABT-102

Bohsen, M. S. (Underviser)

Aktivitet: Foredrag og mundtlige bidragKonferenceoplæg

Beskrivelse

This study investigates the supramolecular assemblies emerging when dispersing an Amorphous Solid Dispersion (ASD) containing the poorly water-soluble drug molecule ABT-102 in aqueous media. A sample containing ASD corresponding to the concentration normally given to patient and a sample containing a modified, lower concentration of ASD were tested. Asymmetric Flow Field-Flow Fractionation (AF4) connected to on-line Multi-Angle Laser Light Scattering (MALLS) was employed to investigate the hydrodynamic size-distribution of submicron assemblies present in the supernatant. In addition, the micrometer sized population in the supernatant, pellet and samples prior to centrifugation were analyzed with Single Particle Optical Sensing (SPOS) to get a full representation of the supramolecular assemblies emerging when dispersing ABT-102 in PBS buffer. Ultra-High Performance Liquid Chromatography (UHPLC) was utilized to quantify ABT-102 in the supernatant and samples prior to centrifugation.
AF4/MALLS showed that three supramolecular assemblies emerged in the ASD dispersions. Triplicates made on three different days showed high reproducibility with the AF4/MALLS set-up. The concentration of ABT-102 was 200 times higher in samples prior centrifugation compared to supernatants. UHPLC showed no significant difference in concentration of ABT-102 in supernatant samples for in vivo and modified concentrations. SPOS results indicate that no particles exceeding 20 μm are emerging.
Periode26. feb. 2020
BegivenhedstitelFFF2020
20th International Symposium on Field- and Flow-Based Separations: null
BegivenhedstypeKonference
PlaceringVienna, Østrig
Grad af anerkendelseInternational