Quantum dot conjugations and single molecule multicolor imaging of plasma membrane proteins and lipids in living cells

Clausen, M. P. (Foredragsholder)

Aktivitet: Foredrag og mundtlige bidragForedrag og præsentationer i privat eller offentlig virksomhed

Beskrivelse

Quantum dots (Qdots) are fluorescent nano-particles with a very high signal intensity and signal stability as compared to conventional fluorescent probes. Qdots are tunable in emission wavelength while all being excitable at the same excitation wavelength. Qdots are available with both primary amines and carboxyl reactive groups on the surface and thereby allows for conjugation of essentially any biomolecule of interest. We have designed, made, and validated Qdots with different biomolecule substrates conjugated to the surfaces for specifically targeting native and artificial tags on plasma membrane proteins and lipids. By using Qdots of different colors imaging can easily be done with simultaneous visualization of up to four different molecular species at single molecule sensitivity and millisecond time integration. We find that this technique is very useful for studying the spatial and temporal nano-organization in the cellular plasma membrane of live mammalian cells.
Emneord: Quantum Dots, Membrane biophysics, Single Particle Tracking, Membrane Organization
Periode31. okt. 2009
Begivenhedstitel10<sup>th</sup> International Summer School on Biophysics
BegivenhedstypeKonference
PlaceringRovinj, Kroatien